Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
文献类型：会议论文
| 作者 | Kang TF(康腾飞) ; Niu Y(牛宇); Jin G(靳刚)
|
| 出版日期 | 2014 |
| 会议名称 | 6th International Conference on Spectroscopic Ellipsometry |
| 会议日期 | MAY 26-31 2013 |
| 会议地点 | Kyoto JAPAN |
| 关键词 | Biosensor Total internal reflection imaging ellipsometry Weak affinity interaction Tris Lysozyme |
| 通讯作者 | gajin@imech.ac.cn |
| 中文摘要 | Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme a typical example of weak affinity interactions has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10 M which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. 2014 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI ; CPCI |
| 会议网址 | http://dx.doi.org/10.1016/j.tsf.2014.02.098 |
| 会议录 | THIN SOLID FILMS
 |
| 会议录出版者 | ELSEVIER SCIENCE SA |
| 会议录出版地 | LAUSANNE |
| 语种 | 英语 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/55365]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Kang TF,Niu Y,Jin G. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[C]. 见:6th International Conference on Spectroscopic Ellipsometry. Kyoto JAPAN. MAY 26-31 2013.http://dx.doi.org/10.1016/j.tsf.2014.02.098. |
入库方式： OAI收割
来源：力学研究所
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