Identification and characterization of MAVS from black carp Mylopharyngodon piceus
文献类型：期刊论文
| 作者 | Zhou, Wei1; Zhou, Jujun1; Lv, Ying1; Qu, Yixiao1; Chi, Mengdie1; Li, Jun1; Feng, Hao1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 43期号:2页码:460-468 |
| 关键词 | Black carp MAVS Innate immunity |
| ISSN号 | 1050-4648 |
| 英文摘要 | MAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate immune response against microbial pathogens. In this study, MAVS has been cloned and characterized from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to mammalian MAVS. bcMAVS is constitutively transcribed in all the selected tissues including gill, kidney, heart, intestine, liver, muscle, skin and spleen; bcMAVS mRNA level in intestine, liver, muscle increased but decreased in spleen right after GCRV or SVCV infection. Multiple bands of bcMAVS were detected in western blot when it was expressed in tissue culture, which is similar to mammalian MAVS. Immunofluorescence assay determined that bcMAVS is a mitochondria protein and luciferase reporter assay demonstrated that bcMAVS could induce zebrafish IFN and EPC IFN expression in tissue culture. Data generated in this manuscript has built a solid foundation for further elucidating the function of bcMAVS in the innate immune system of black carp. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | ANTIVIRAL SIGNALING PROTEIN ; TOLL-LIKE RECEPTORS ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; ADAPTER PROTEIN ; INNATE IMMUNITY ; RNA HELICASE ; TELEOST FISH ; HOST-DEFENSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352923700018 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27115]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Minist Educ China, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China 2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Wei,Zhou, Jujun,Lv, Ying,et al. Identification and characterization of MAVS from black carp Mylopharyngodon piceus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):460-468. |
| APA | Zhou, Wei.,Zhou, Jujun.,Lv, Ying.,Qu, Yixiao.,Chi, Mengdie.,...&Feng, Hao.(2015).Identification and characterization of MAVS from black carp Mylopharyngodon piceus.FISH & SHELLFISH IMMUNOLOGY,43(2),460-468. |
| MLA | Zhou, Wei,et al."Identification and characterization of MAVS from black carp Mylopharyngodon piceus".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):460-468. |
入库方式： OAI收割
来源：水生生物研究所
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