Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7
文献类型：期刊论文
| 作者 | Lu, Long-Feng1,2; Li, Shun1; Lu, Xiao-Bing1,2; Zhang, Yong-An1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 45期号:2页码:574-582 |
| 关键词 | MAVS variants Negative regulator Interferon IRF7 Zebrafish |
| ISSN号 | 1050-4648 |
| 英文摘要 | IFNs create the first line of host cells to defense viral infection, however, unrestricted expression of IFN can be hazardous to the host. IRF7 is the master regulator of type I IFN expression. To our knowledge, non research about the inhibition of IFN expression by targeting IRF7 has been reported in fish. In this study, we reported that the splicing variant of wildtype MAVS (MAVS_tv1), MAVS_tv2, negatively regulated IRF7-mediated IFN production. Firstly, in vivo, the transcriptional levels of MAVS_tv2 in trunk kidney and spleen from the zebrafish infected with SVCV were monitored. Then, in vitro, the protein expression pattern of MAVS_tv2 in zebrafish cell lines was detected using anti-MAVS_tv2 antibody. Furthermore, overexpression of MAVS_tv2 decreased the activation of IFNI promoter that induced by IRF7 in a dose-dependent manner, whereas it had little effect on IRF3, a close relative of IRF7. In addition, such inhibition was also observed in IRF7-mediated epcIFN promoter and ISRE activities, but not in the activation of the promoters of type II IFNs and NF-kappa B, due to IRF7 not regulating their expression. Lastly, overexpression of MAVS_tv2 decreased the transcriptional levels of several IFN-stimulated genes activated by IRF7. These findings suggest that MAVS_tv2 is a negative regulator of IFNI by targeting IRF7. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSE ; TOLL-LIKE RECEPTORS ; HEPATITIS-C-VIRUS ; NF-KAPPA-B ; RIG-I ; PATHOGEN RECOGNITION ; NEGATIVE REGULATION ; SPLICING VARIANTS ; ADAPTER PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358626800043 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/25972]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Long-Feng,Li, Shun,Lu, Xiao-Bing,et al. Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):574-582. |
| APA | Lu, Long-Feng,Li, Shun,Lu, Xiao-Bing,&Zhang, Yong-An.(2015).Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7.FISH & SHELLFISH IMMUNOLOGY,45(2),574-582. |
| MLA | Lu, Long-Feng,et al."Functions of the two zebrafish MAVS variants are opposite in the induction of IFN1 by targeting IRF7".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):574-582. |
入库方式： OAI收割
来源：水生生物研究所
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