Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation
文献类型：期刊论文
| 作者 | Li, Wen-Hua1,2; Zhou, Li1; Wang, Yang1; Shi, Jian-Tao1; Yang, Yan-Jing1,2; Gui, Jian-Fang1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
 |
| 出版日期 | 2015 |
| 卷号 | 11期号:6页码:688-700 |
| 关键词 | Lbh-like retina development photoreceptor differentiation otx2 Notch |
| ISSN号 | 1449-2288 |
| 英文摘要 | The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | TRANSCRIPTIONAL REGULATOR LBH ; HOMEOBOX GENE OTX2 ; RETINAL STEM-CELLS ; SIGNALING PATHWAY ; PROMOTES RETINOGENESIS ; EMBRYONIC-DEVELOPMENT ; NEURONAL PROGENITORS ; MAMMALIAN RETINA ; COFACTOR LBH ; MULLER GLIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359007300008 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/25976]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wen-Hua,Zhou, Li,Wang, Yang,et al. Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2015,11(6):688-700. |
| APA | Li, Wen-Hua,Zhou, Li,Wang, Yang,Shi, Jian-Tao,Yang, Yan-Jing,&Gui, Jian-Fang.(2015).Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,11(6),688-700. |
| MLA | Li, Wen-Hua,et al."Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 11.6(2015):688-700. |
入库方式： OAI收割
来源：水生生物研究所
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