Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR
文献类型：期刊论文
| 作者 | Chen, W; Song, LR; Ou, DY; Gan, NQ |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 20期号:3页码:323-330 |
| ISSN号 | 1520-4081 |
| 关键词 | Daphnia magna microcystin chronic toxicities ultrastructure enzyme |
| 英文摘要 | In the current study, the toxicological mechanisms of microcystin-LR and its disadvantageous effects on Daphnia magna were examined. Survival rate, number of newborn, activity of several important enzymes [glutathione S-transferase (GST), lactate dehydrogenase (LDH), phosphatases, and glutathione], accumulated microcystins, and ultrastructural changes in different organs of Daphnia were monitored over the course of 21-day chronic tests. The results indicated that low concentrations of dissolved microcystin had no harmful effect on Daphnia. On the contrary, stimulatory effects were detected. In the presence of toxin at high dosage and for long-term exposure, GST and glutathione levels decreased significantly. The decreased enzyme activity in the antioxidant system probably was caused by detoxification reactions with toxins. And these processes of detoxification at the beginning of chronic tests may enable phosphatases in Daphnia magna to withstand inhibition by the toxins. At the same time, we also found that the LDH activity in test animals increased with exposure to microcystin-LR, indicating that adverse effects occurred in Daphnia. With microcystin given at a higher dosage or for a longer exposure, the effect on Daphnia magna was fatal. In the meantime, microcystin began to accumulate in Daphnia magna, and phosphatase activity started to be inhibited. From the ultrastructure results of cells in D. magna, we obtained new information: the alimentary canal may be the target organ affected by exposure of microcystins to D. magna. The results of the current study also suggested that the oxidative damage and PPI (protein phosphatase inhibition) mechanisms of vertebrates also are adapted to Daphnia. (c) 2005 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | CULTURED RAT HEPATOCYTES ; CARASSIUS-AURATUS L ; CYANOBACTERIA ; GLUTATHIONE ; AERUGINOSA ; CHROMATOGRAPHY ; VARIANTS ; BINDING ; TOXINS |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229897500016 |
| 公开日期 | 2015-09-29 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/25899]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, W,Song, LR,Ou, DY,et al. Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR[J]. ENVIRONMENTAL TOXICOLOGY,2005,20(3):323-330. |
| APA | Chen, W,Song, LR,Ou, DY,&Gan, NQ.(2005).Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR.ENVIRONMENTAL TOXICOLOGY,20(3),323-330. |
| MLA | Chen, W,et al."Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR".ENVIRONMENTAL TOXICOLOGY 20.3(2005):323-330. |
入库方式： OAI收割
来源：水生生物研究所
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