Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics
文献类型：期刊论文
| 作者 | Xiong, Qian2; Zhong, Qiu3; Zhang, Jia1,2; Yang, Mingkun2; Li, Chongyang2; Zheng, Peng2; Bi, Li-Jun1; Ge, Feng2 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 11期号:4页码:2078-2090 |
| 关键词 | microRNA-21 (miR-21) multiple myeloma (MM) stable isotope labeling by amino acids in cell culture (SILAC) signal transducer and activator of transcription 3 (STAT3) protein inhibitor of activated STAT3 (PIAS3) |
| ISSN号 | 1535-3893 |
| 英文摘要 | Substantial evidence indicates that microRNA-21 (miR-21) is a key oncomiR in carcinogenesis and is significantly elevated in multiple myeloma (MM). In this study, we explored the role of miR-21 in human MM cells and searched for miR-21 targets. By knocking down the expression of endogenous miR-21 in U266 myeloma cells, we observed reduced growth, an arrested cell cycle, and increased apoptosis. To further understand its molecular mechanism in the pathogenesis of MM, we employed a SILAC (stable isotope labeling by amino acids in cell culture)-based quantitative proteomic strategy to systematically identify potential targets of miR-21. In total, we found that the expression of 178 proteins was up-regulated significantly by miR-21 inhibition, implying that they could be potential targets of miR-21. Among these, the protein inhibitor of activated STAT3 (PIAS3) was confirmed as a direct miR-21 target by Western blotting and reporter gene assays. We further demonstrated that miR-21 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3 and that down-regulation of PIAS3 contributes to the oncogenic function of miR-21. This elucidation of the role of PIAS3 in the miR-21-STAT3 positive regulatory loop not only may shed light on the molecular basis of the biological effects of miR-21 observed in MM cells but also has direct implications for the development of novel anti-MM therapeutic strategies. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HUMAN GLIOBLASTOMA CELLS ; MICRORNA EXPRESSION ; GENE-EXPRESSION ; STAT3 INHIBITOR ; DOWN-REGULATION ; TUMOR-GROWTH ; PIAS3 ; ACTIVATION ; CANCER ; TRANSCRIPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302388100006 |
| 公开日期 | 2015-10-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27124]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Noncoding RNA, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.AntiTB Res Inst Guangdong Prov, IBPARI Joint Ctr Res TB, Guangzhou 510630, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Qian,Zhong, Qiu,Zhang, Jia,et al. Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(4):2078-2090. |
| APA | Xiong, Qian.,Zhong, Qiu.,Zhang, Jia.,Yang, Mingkun.,Li, Chongyang.,...&Ge, Feng.(2012).Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics.JOURNAL OF PROTEOME RESEARCH,11(4),2078-2090. |
| MLA | Xiong, Qian,et al."Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics".JOURNAL OF PROTEOME RESEARCH 11.4(2012):2078-2090. |
入库方式： OAI收割
来源：水生生物研究所
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