Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques
文献类型：期刊论文
| 作者 | Li, Huan1; Zhong, Qiuping1; Wirth, Stephan2; Wang, Weiwei1; Hao, Yaotong1; Wu, Shangong1; Zou, Hong1; Li, Wenxiang1; Wang, Guitang1 |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 46期号:10页码:2344-2359 |
| 关键词 | Grass carp autochthonous microbiota gut mucosa 16S rDNA |
| ISSN号 | 1355-557X |
| 英文摘要 | Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and Deinococcus-Thermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C-s < 0.80), but gamma-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries |
| 研究领域[WOS] | Fisheries |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; COMPLETE GENOME SEQUENCE ; 16S RIBOSOMAL-RNA ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; MICROBIAL DIVERSITY ; GROWTH-PERFORMANCE ; GUT MICROBIOTA ; AUREUS MALE ; FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360671700004 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27174]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Muncheberg, Germany |
| 推荐引用方式 GB/T 7714 | Li, Huan,Zhong, Qiuping,Wirth, Stephan,et al. Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques[J]. AQUACULTURE RESEARCH,2015,46(10):2344-2359. |
| APA | Li, Huan.,Zhong, Qiuping.,Wirth, Stephan.,Wang, Weiwei.,Hao, Yaotong.,...&Wang, Guitang.(2015).Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques.AQUACULTURE RESEARCH,46(10),2344-2359. |
| MLA | Li, Huan,et al."Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques".AQUACULTURE RESEARCH 46.10(2015):2344-2359. |
入库方式： OAI收割
来源：水生生物研究所
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