Magic angle spinning NMR and H-1-P-31 heteronuclear statistical total correlation spectroscopy of intact human gut biopsies
文献类型：期刊论文
| 作者 | Wang, Yulan1; Cloarec, Olivier1; Tang, Huiru2; Lindon, John C.1; Noimes, Elaine1; Kochhar, Sunil3; Nicholson, Jeremy K.1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008-02-15 |
| 卷号 | 80期号:4页码:1058-1066 |
| 英文摘要 | Previously we have demonstrated the use of H-1 magic angle spinning (MAS) NMR spectroscopy for the topographical variations in functional metabolic signatures of intact human intestinal biopsy samples. Here we have analyzed a series of MAS H-1 NMR spectra (spin-echo, one-dimensional, and diffusion-edited) and P-31-{H-1} spectra and focused on analyzing the enhancement of information recovery by use of the statistical total correlation spectroscopy (STOCSY) method. We have applied a heterospectroscopic cross-examination performed on the same samples and between H-1 and P-31-{H-1} spectra (heteronuclear STOCSY) to recover latent metabolic information. We show that heterospectroscopic correlation can give new information on the molecular compartmentation of metabolites in intact tissues, including the statistical "isolation" of a phosphlipid/triglyceride vesicle pool in intact tissue. The application of P-31-H-1 HET-STOCSY allowed the cross-assignment of major P-31 Signals to their equivalent H-1 NMR spectra, e.g., for phosphorylcholine and phosphorylethanolamine. We also show pathway correlations, e.g., the ascorbate-glutathione pathway, in the STOCSY analysis of intact tissue spectra. These P-31-H-1 HET-STOCSY spectra also showed different topographical regions, particular for minor signals in different tissue microenvironments. Ibis approach could be extended to allow the detection of altered distributions within metabolic subcompartments as well as conventional metabonomics concentration-based diagnostics. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; H-1-NMR SPECTROSCOPY ; BIOMARKER IDENTIFICATION ; PATTERN-RECOGNITION ; DATA SETS ; METABONOMICS ; METABOLISM ; TAURINE ; URINARY ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253165400020 |
| 公开日期 | 2015-10-13 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8000]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Biomol Med, SORA Div, London SW7 2AZ, England 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol, Wuhan 430071, Peoples R China 3.Nestec Ltd, Nestle Res Ctr, Vers Chez Less Blanc, CH-1000 Lausanne, Switzerland |
| 推荐引用方式 GB/T 7714 | Wang, Yulan,Cloarec, Olivier,Tang, Huiru,et al. Magic angle spinning NMR and H-1-P-31 heteronuclear statistical total correlation spectroscopy of intact human gut biopsies[J]. ANALYTICAL CHEMISTRY,2008,80(4):1058-1066. |
| APA | Wang, Yulan.,Cloarec, Olivier.,Tang, Huiru.,Lindon, John C..,Noimes, Elaine.,...&Nicholson, Jeremy K..(2008).Magic angle spinning NMR and H-1-P-31 heteronuclear statistical total correlation spectroscopy of intact human gut biopsies.ANALYTICAL CHEMISTRY,80(4),1058-1066. |
| MLA | Wang, Yulan,et al."Magic angle spinning NMR and H-1-P-31 heteronuclear statistical total correlation spectroscopy of intact human gut biopsies".ANALYTICAL CHEMISTRY 80.4(2008):1058-1066. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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