Correlation between choline signal intensity and acetylcholine level in different brain regions of rat
文献类型：期刊论文
| 作者 | Wang, Xiao-Chuan1,2; Du, Xiao-Xia3; Tian, Qing1,2; Wang, Jian-Zhi1,2 |
| 刊名 | NEUROCHEMICAL RESEARCH
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 33期号:5页码:814-819 |
| 关键词 | choline-containing compounds acetylcholine H-1 MRS microdialysis high-performance liquid chromatography |
| 英文摘要 | Acetylcholine is an important excitatory neurotransmitter, which plays a crucial role in synaptic transmission. The level of acetylcholine is decreased in the early stages of Alzheimer disease (AD), the most common neurodegenerative disease. Therefore, measurement of acetylcholine in the brain may help the clinical diagnosis of AD. However, the methods used till now to detect the brain acetylcholine level are invasive, which are neither recommended nor acceptable in the clinic. Acetylcholine is synthesized from choline-containing compounds (Cho), the latter can be estimated by noninvasive proton magnetic resonance spectroscopy (H-1 MRS). To explore whether the Cho signal intensity could be used to represent the acetylcholine level in the brain, we employed H-1 MRS to detect the Cho signal, and simultaneously, we also used microdialysis and high-performance liquid chromatography (HPLC) to measure the level of acetylcholine in hippocampus, striatum, frontal cortex, and somatosensory barrel field (S1BF cortex) of rats, respectively. The results showed that the correlations between Cho signal intensity and acetylcholine level in hippocampus, striatum, frontal cortex, and S1BF cortex were, respectively, 0.823 (p = 0.044), 0.851 (p = 0.032), 0.817 (p = 0.047), and 0.822 (p = 0.045). The F-values of the regression model were, respectively, 8.404 (p = 0.044), 10.47 (p = 0.032), 8.000 (p = 0.047), and 8.326 (p = 0.045). And the derived regression equations were y = 0.67x + 1.363 (hippocampus), y = 5.398x + 6.684 (striatum), y = 0.656x + 0.564 (frontal cortex), and y = 0.394x + 1.127 (S1BF cortex), respectively (y means acetylcholine, and x means Cho). These data suggest that the Cho signal intensity observed by H-1 MRS may be used as an indicator of acetylcholine level in different brain regions of the rats. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; PAIRED HELICAL FILAMENTS ; FREELY MOVING RATS ; ALZHEIMERS-DISEASE ; L-CARNITINE ; BASAL FOREBRAIN ; PROTEIN-TAU ; NEURONS ; RELEASE ; SYSTEMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254238300012 |
| 公开日期 | 2015-10-13 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8038]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Pathophysiol, Inst Neurosci, Wuhan 430030, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Hubei Prov Key Lab Neurol Dis, Wuhan 430030, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiao-Chuan,Du, Xiao-Xia,Tian, Qing,et al. Correlation between choline signal intensity and acetylcholine level in different brain regions of rat[J]. NEUROCHEMICAL RESEARCH,2008,33(5):814-819. |
| APA | Wang, Xiao-Chuan,Du, Xiao-Xia,Tian, Qing,&Wang, Jian-Zhi.(2008).Correlation between choline signal intensity and acetylcholine level in different brain regions of rat.NEUROCHEMICAL RESEARCH,33(5),814-819. |
| MLA | Wang, Xiao-Chuan,et al."Correlation between choline signal intensity and acetylcholine level in different brain regions of rat".NEUROCHEMICAL RESEARCH 33.5(2008):814-819. |
入库方式： OAI收割
来源：武汉物理与数学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。