Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model
文献类型：期刊论文
| 作者 | Zhu, Wen-Zhen2; Li, Xiang2; Qi, Jian-Pin2; Tang, Zhou-Ping1; Wang, Wei1; Wei, Li3; Lei, Hao3 |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 21期号:5页码:420-424 |
| 关键词 | Stein cell transplantation Magnetic resonance imaging Staining and Labelling Cell migration Cell differentiation |
| 英文摘要 | Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide (SPIO) and bromodeoxyuridine (Brdu) using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells (NSCs)co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine (BrdU) were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion (MCAO). At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6, the rats were killed and their brain tissue was cut according to the migration site of transplanted cells indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusions MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Public, Environmental & Occupational Health |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 关键词[WOS] | IN-VIVO ; MRI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260448100009 |
| 公开日期 | 2015-10-13 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8072]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Neurol, Wuhan 430030, Hubei, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Radiol, Wuhan 430030, Hubei, Peoples R China 3.Chinese Acad Sci, WIPM, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430030, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Wen-Zhen,Li, Xiang,Qi, Jian-Pin,et al. Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2008,21(5):420-424. |
| APA | Zhu, Wen-Zhen.,Li, Xiang.,Qi, Jian-Pin.,Tang, Zhou-Ping.,Wang, Wei.,...&Lei, Hao.(2008).Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,21(5),420-424. |
| MLA | Zhu, Wen-Zhen,et al."Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 21.5(2008):420-424. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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