Assessment of Amino Acid Neurotransmitters in Rat Brain Microdialysis Samples by High-Performance Liquid Chromatography with Coulometric Detection
文献类型：期刊论文
| 作者 | Chen, Rongxiang1,3; Deng, Yinghua1,4; Yao, Jiao4; Kamal, Ghulam Mustafa1,3; Wang, Jie1; Xu, Fuqiang1,2 |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2015-09-14 |
| 卷号 | 38期号:15页码:1439-1447 |
| 关键词 | coulometric detection awake animal high-performance liquid chromatography microdialysis olfactory bulb amino acid neurotransmitters |
| 英文摘要 | A simple method was developed for simultaneous determination of five amino acid neurotransmitters (taurine, glutamate, aspartate, glycine, and gamma-aminobutyric acid) in brain microdialysates by high-performance liquid chromatography with coulometric detection. The microdialysates were derivatized with o-phthalaldehyde in 5mM sodium sulfite and separated within 17min using isocratic elution. Detection parameters for these transmitters were linearity (r(2))>0.999 over the concentration range from 0.1 to 15.00 mu M (0.01-1.00 mu M for GABA); reproducibility 2.88%-11.67% (relative standard deviation); the limits of detection 4.4-17.2 fmol; and recovery 86.66%-103.42%. This method was successfully applied to analyze these neurotransmitters in the brain. The changes of neurotransmitters were also investigated via local administration of potassium chloride and bicuculline, which was a gamma-aminobutyric acid receptor antagonist. It was found that the developed method is more efficient as compared to previously reported methods in simultaneously determining five major neurotransmitters because it shows quick separation and sensitive detection, and is easy to operate. This method can be a practical choice for continuous monitoring of the amino acid neurotransmittersin vivo. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; IN-VIVO MICRODIALYSIS ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; OLFACTORY-BULB ; SULFITE DERIVATIZATION ; FLUORESCENCE DETECTION ; PARKINSONS-DISEASE ; MUSCLE-CONTRACTION ; MASS-SPECTROMETRY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360437800001 |
| 公开日期 | 2015-10-13 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/7877]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 2.Wuhan Natl Lab Optoelect, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Hubei Univ Educ, Dept Chem & Life Sci, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Rongxiang,Deng, Yinghua,Yao, Jiao,et al. Assessment of Amino Acid Neurotransmitters in Rat Brain Microdialysis Samples by High-Performance Liquid Chromatography with Coulometric Detection[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(15):1439-1447. |
| APA | Chen, Rongxiang,Deng, Yinghua,Yao, Jiao,Kamal, Ghulam Mustafa,Wang, Jie,&Xu, Fuqiang.(2015).Assessment of Amino Acid Neurotransmitters in Rat Brain Microdialysis Samples by High-Performance Liquid Chromatography with Coulometric Detection.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,38(15),1439-1447. |
| MLA | Chen, Rongxiang,et al."Assessment of Amino Acid Neurotransmitters in Rat Brain Microdialysis Samples by High-Performance Liquid Chromatography with Coulometric Detection".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38.15(2015):1439-1447. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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