Immobilization of trypsin onto multifunctional meso-fmacroporous core-shell microspheres: A new platform for rapid enzymatic digestion
文献类型：期刊论文
| 作者 | Cheng, Gong ; Chen, Ping ; Wang, Zhi-Gang ; Sui,Xiao-Jing ; Zhang,Ji-Lin ; Ni,Jia-Zan |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2014 |
| 卷号 | 812期号:2页码:65-73 |
| 关键词 | MAGNETIC NANOPARTICLES SELECTIVE ENRICHMENT PROTEIN DIGESTION MEMBRANE-PROTEINS PROTEOMICS PROTEOLYSIS EFFICIENT ENZYMES IDENTIFICATION SEPARATION |
| 通讯作者 | zhang,jl |
| 英文摘要 | a simple, fast, efficient, and reusable microwave-assisted tryptic digestion system which was constructed by immobilization of trypsin onto porous core-shell fe304@ftio2 microspheres has been developed. the nanostructure with magnetic core and titania shell has multiple pore sizes (2.4 and 15.0 nm), high pore volume (0.25 cm3 g-1), and large surface area (50.45 m2 g-1). for the proteins, the system can realize fast and efficient microwave-assisted tryptic digestion. various standard proteins (e.g., cytochrome c (cytc), myoglobin (myo), p-lactoglobulin (p-lg), and bovine serum albumin (bsa)) used can be digested in 45s under microwave radiation, and they can be confidently identified by mass spectrometry (ms) analysis; even the concentration of substrate is as low as 5 ng pi -1. furthermore, the system for the 45s microwave-assisted tryptic digestion is still effective after the trypsin-immobilized microspheres have been reused for 5 times. importantly, 1715 unique proteins from 10 tig mouse brain proteins can be identified with high confidence after treatment of 45 s microwave-assisted tryptic digestion. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-10-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/56248]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cheng, Gong,Chen, Ping,Wang, Zhi-Gang,et al. Immobilization of trypsin onto multifunctional meso-fmacroporous core-shell microspheres: A new platform for rapid enzymatic digestion[J]. analytica chimica acta,2014,812(2):65-73. |
| APA | Cheng, Gong,Chen, Ping,Wang, Zhi-Gang,Sui,Xiao-Jing,Zhang,Ji-Lin,&Ni,Jia-Zan.(2014).Immobilization of trypsin onto multifunctional meso-fmacroporous core-shell microspheres: A new platform for rapid enzymatic digestion.analytica chimica acta,812(2),65-73. |
| MLA | Cheng, Gong,et al."Immobilization of trypsin onto multifunctional meso-fmacroporous core-shell microspheres: A new platform for rapid enzymatic digestion".analytica chimica acta 812.2(2014):65-73. |
入库方式： OAI收割
来源：长春应用化学研究所
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