On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection
文献类型：期刊论文
| 作者 | Li, Libo ; Xu, Lei ; Huang, Jianshe ; You,Tianyan |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2014 |
| 卷号 | 331期号:3页码:117-122 |
| 关键词 | Capillary electrophoresis Amperometric detection Field-enhanced sample injection Dynamic pH junction 5-HT3 receptor antagonists |
| 通讯作者 | you,ty |
| 英文摘要 | in the present work, an on-line dual focusing technique based on field-enhance sample injection (fasi) and dynamic ph junction (dyph) was developed for the analysis of two 5-hydroxy-tryptamine type 3 receptor (5-ht3) antagonists ondansetron (ond) and tropisetron (tro) by capillary electrophoresis with amperometric detection (ce-ad) system. by preparing the sample in a lower conductivity (fasi condition) and lower ph value (dyph condition) matrix relative to the background electrolyte (bge) solution, a simple and effective dual focusing approach, fasi-dyph was achieved. in this stacking mode, a large amount of analytes could be electrokinetically injected into the capillary and stacked at the boundary of the sample and the bge zone as a result of deprotonation and decrease in the electric field. effects of separation, detection and fasi-dyph focusing conditions were investigated in detail. under the optimum conditions, good separation for ond and tro was achieved within 8 min. in comparison with the conventional ce-ad analysis method, the present dual focusing technique enabled the enhancement factors in terms of peak heights to reach 357-fold and 345-fold for ond and tro, respectively. the limits of detection (lods) (s/n =3) for ond and tro were 2 nm and 5 nm, respectively. the intraday and interday repeatabilities (rsds) were less than 4.5% and 2.9% for peak height and migration time, respectively. the proposed method was successfully applied for the analysis of ond and tro in human urine sample. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-10-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57201]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, Libo,Xu, Lei,Huang, Jianshe,et al. On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection[J]. journal of chromatography a,2014,331(3):117-122. |
| APA | Li, Libo,Xu, Lei,Huang, Jianshe,&You,Tianyan.(2014).On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection.journal of chromatography a,331(3),117-122. |
| MLA | Li, Libo,et al."On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection".journal of chromatography a 331.3(2014):117-122. |
入库方式： OAI收割
来源：长春应用化学研究所
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