Exploring the interaction of cisplatin with beta 2-microglobulin: new insights into a chemotherapeutic drug
文献类型：期刊论文
| 作者 | Zhang,Ningbo ; Cui,Meng ; Du,Yonggang ; Liu,Zhiqiang ; Liu,Shuying |
| 刊名 | rsc advances
 |
| 出版日期 | 2014 |
| 卷号 | 4期号:5页码:2300-2305 |
| 关键词 | RESONANCE MASS-SPECTROMETRY DIALYSIS-RELATED AMYLOIDOSIS COPPER CHAPERONE ATOX1 ZINC-FINGER PROTEIN TOP-DOWN SUPEROXIDE-DISMUTASE SERUM-PROTEINS BINDING-SITES CROSS-LINKING CYTOCHROME-C |
| 通讯作者 | cui,m |
| 英文摘要 | cisplatin is one of the most widely used anticancer platinum drugs and the interactions between cisplatin and proteins are deemed to be associated with its severe side-effects and drug resistance generated in the chemotherapy process. here, the interaction of cisplatin with beta 2-microglobulin was investigated using electrospray ionization fourier transform ion cyclotron resonance mass spectrometry (esi fticr ms). high resolution ms data revealed that b2-microglobulin (beta 2m) can quickly react with cisplatin in 5 mm nh4oac aqueous solution (ph 7.0) and up to five platinated complexes were bound to beta 2m in 24 h. the primary monoadduct was determined to be beta 2-microglobulin-pt(nh3). besides, the oxidation of met99 to methionine sulfoxide decreased the reaction velocity of beta 2m and cisplatin. four binding sites for cisplatin to beta 2m have been identified using fticr ms combined with a bottom-up approach without complex liquid chromatography separation. met99 and his51 were identified as the main binding sites. besides, his84 and his31 were the other two binding sites. what's more, the platination of beta 2m disrupts the conformation of the protein, and coordination of different platinum species induced different conformation changes of beta 2m. interestingly, a pt(ii) bridged dimer of beta 2m was observed under physiological conditions. this work highlights the ability of high resolution mass spectrometry to study the interactions between beta 2m and cisplatin, but also provides unique insights into the mechanism of side-effects of cisplatin at a molecular level. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329035400029 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57246]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang,Ningbo,Cui,Meng,Du,Yonggang,et al. Exploring the interaction of cisplatin with beta 2-microglobulin: new insights into a chemotherapeutic drug[J]. rsc advances,2014,4(5):2300-2305. |
| APA | Zhang,Ningbo,Cui,Meng,Du,Yonggang,Liu,Zhiqiang,&Liu,Shuying.(2014).Exploring the interaction of cisplatin with beta 2-microglobulin: new insights into a chemotherapeutic drug.rsc advances,4(5),2300-2305. |
| MLA | Zhang,Ningbo,et al."Exploring the interaction of cisplatin with beta 2-microglobulin: new insights into a chemotherapeutic drug".rsc advances 4.5(2014):2300-2305. |
入库方式： OAI收割
来源：长春应用化学研究所
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