Comprehensive identification of the binding sites of cisplatin in hen egg white lysozyme
文献类型：期刊论文
| 作者 | Zhang, Ningbo ; Du, Yonggang ; Cui, Meng ; Liu,Zhiqiang ; Liu,Shuying |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 406期号:15页码:3537-3549 |
| 关键词 | RESONANCE MASS-SPECTROMETRY ANTICANCER DRUG CISPLATIN AQUEOUS SOLVENT SYSTEMS X-RAY-DIFFRACTION TOP-DOWN CARBOPLATIN BINDING CHAPERONE ATOX1 CYTOCHROME-C PROTEIN ESI |
| 通讯作者 | cui,m |
| 英文摘要 | platinum drugs have become one of the most important kinds of chemotherapy agents, and the interactions of these drugs with proteins play very important roles in their side effects and drug resistance. however, it is still a challenge to determine the binding sites of platinum drugs in proteins with multiple disulfide bonds and stable three-dimensional structures using mass spectrometry. here, the interaction between cisplatin and hen egg white lysozyme (hewl), a multi-disulfide-bond-containing protein with a stable three-dimensional structure, was investigated using fourier transform ion cyclotron resonance mass spectrometry. typical disulfide bond reduction with dithiothreitol/tris(2-carboxyethyl)phosphine before trypsin digestion destroyed the binding of cisplatin to hewl, and no platination sites were found. efficient trypsin digestion methods for hewl-cisplatin adducts were developed to avoid the loss of platinum binding to protein. at 55 a degrees c, platinated hewl was digested directly by trypsin in 6 h, and multiple platinated peptides were observed. in 60 % acetonitrile, the digestion time of platinated hewl was shortened to 2 h, and most of the platinated peptides were observed. in addition, the reduction of the disulfide bonds of hewl greatly accelerated the reaction between hewl and cisplatin, and the potential binding sites of cisplatin in reduced hewl could be easily recognized. on the basis of the above-mentioned methods, multiple binding sites of cisplatin in hewl were first identified by mass spectrometry. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336393900003 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57329]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, Ningbo,Du, Yonggang,Cui, Meng,et al. Comprehensive identification of the binding sites of cisplatin in hen egg white lysozyme[J]. analytical and bioanalytical chemistry,2014,406(15):3537-3549. |
| APA | Zhang, Ningbo,Du, Yonggang,Cui, Meng,Liu,Zhiqiang,&Liu,Shuying.(2014).Comprehensive identification of the binding sites of cisplatin in hen egg white lysozyme.analytical and bioanalytical chemistry,406(15),3537-3549. |
| MLA | Zhang, Ningbo,et al."Comprehensive identification of the binding sites of cisplatin in hen egg white lysozyme".analytical and bioanalytical chemistry 406.15(2014):3537-3549. |
入库方式： OAI收割
来源：长春应用化学研究所
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