Rapid screening and detection of XOD inhibitors from S-tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC
文献类型：期刊论文
| 作者 | Wang, Jing ; Liu, Shu ; Ma, Bing ; Chen,Lina ; Song,Fengrui ; Liu,Zhiqiang ; Liu,Chun-ming |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2014 |
| 卷号 | 406期号:28页码:7379-7387 |
| 关键词 | COUNTER-CURRENT CHROMATOGRAPHY XANTHINE-OXIDASE INHIBITORS MASS-SPECTROMETRY FLAVONOIDS LEAVES HPLC BIFLAVONOIDS RISK GOUT MEN |
| 通讯作者 | liu,s |
| 英文摘要 | xanthine oxidase (xod) catalyzes the metabolism of hypoxanthine and xanthine to uric acid, the overproduction of which could cause hyperuricemia, a risk factor for gout. inhibition of xod is a major treatment for gout, and biflavonoids have been found to act as xod-inhibitory compounds. in this study, ultrafiltration liquid chromatography with photodiode-array detection coupled to electrospray-ionization tandem mass spectrometry (uf-lc-pda-esi-ms) was used to screen and identify xod inhibitors from s. tamariscina. high-performance counter-current chromatography (hpccc) was used to separate and isolate the active constituents of these xod inhibitors. furthermore, ultrahigh-performance liquid chromatography (uplc) and triple-quadrupole mass spectrometry (tq-ms) was used to determine the xod-inhibitory activity of the obtained xod inhibitors, and enzyme kinetics was performed with lineweaver-burk (lb) plots using xanthine as the substrate. as a result, two compounds in s. tamariscina were screened as xod inhibitors: 65.31 mg amentoflavone and 0.76 mg robustaflavone were isolated from approximately 2.5 g s. tamariscina by use of hpccc. the purities of the two compounds obtained were over 98 % and 95 %, respectively, as determined by high-performance liquid chromatography (hplc). lineweaver-burk plot analysis indicated that amentoflavone and robustaflavone were non-competitive inhibitors of xod, and the ic (50) values of amentoflavone and robustaflavone for xod inhibition were 16.26 mu g ml(-1) (30.22 mu mol l-1) and 11.98 mu g ml(-1) (22.27 mu mol l-1), respectively. the ic (50) value of allopurinol, used as the standard, was 7.49 mu g ml(-1) (46.23 mu mol l-1). the results reveal that the method for systematic screening, identification, and isolation of bioactive components in s. tamariscina and for detecting their inhibitory activity using ultrafiltration lc-esi-ms, hpccc, and uplc-tq-ms is feasible and efficient, and could be expected to extend to screening and separation of other enzyme inhibitors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-10-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57330]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Liu, Shu,Ma, Bing,et al. Rapid screening and detection of XOD inhibitors from S-tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC[J]. analytical and bioanalytical chemistry,2014,406(28):7379-7387. |
| APA | Wang, Jing.,Liu, Shu.,Ma, Bing.,Chen,Lina.,Song,Fengrui.,...&Liu,Chun-ming.(2014).Rapid screening and detection of XOD inhibitors from S-tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC.analytical and bioanalytical chemistry,406(28),7379-7387. |
| MLA | Wang, Jing,et al."Rapid screening and detection of XOD inhibitors from S-tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC".analytical and bioanalytical chemistry 406.28(2014):7379-7387. |
入库方式： OAI收割
来源：长春应用化学研究所
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