Studying the Nucleated Mammalian Cell Membrane by Single Molecule Approaches
文献类型：期刊论文
| 作者 | Zhao, Weidong ; Tian, Yongmei ; Cai, Mingjun ; Wang,Feng ; Wu,Jiazhen ; Gao,Jing ; Liu,Shuheng ; Jiang,Junguang ; Jiang,Shibo ; Wang,Hongda |
| 刊名 | plos one
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:5页码:5057-5115 |
| 关键词 | ATOMIC-FORCE MICROSCOPY SUPERRESOLUTION FLUORESCENCE MICROSCOPY OPTICAL RECONSTRUCTION MICROSCOPY FLUID MOSAIC MODEL CRYSTAL-STRUCTURE PLASMA-MEMBRANE LIPID RAFTS RECEPTOR MICRODOMAINS PROTEINS |
| 通讯作者 | jiang,sb |
| 英文摘要 | the cell membrane plays a key role in compartmentalization, nutrient transportation and signal transduction, while the pattern of protein distribution at both cytoplasmic and ectoplasmic sides of the cell membrane remains elusive. using a combination of single-molecule techniques, including atomic force microscopy (afm), single molecule force spectroscopy (smfs) and stochastic optical reconstruction microscopy (storm), to study the structure of nucleated cell membranes, we found that (1) proteins at the ectoplasmic side of the cell membrane form a dense protein layer (4 nm) on top of a lipid bilayer; (2) proteins aggregate to form islands evenly dispersed at the cytoplasmic side of the cell membrane with a height of about 10-12 nm; (3) cholesterol-enriched domains exist within the cell membrane; (4) carbohydrates stay in microdomains at the ectoplasmic side; and (5) exposed amino groups are asymmetrically distributed on both sides. based on these observations, we proposed a protein layer-lipid-protein island (pllpi) model, to provide a better understanding of cell membrane structure, membrane trafficking and viral fusion mechanisms. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335728900003 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57357]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhao, Weidong,Tian, Yongmei,Cai, Mingjun,et al. Studying the Nucleated Mammalian Cell Membrane by Single Molecule Approaches[J]. plos one,2014,9(5):5057-5115. |
| APA | Zhao, Weidong.,Tian, Yongmei.,Cai, Mingjun.,Wang,Feng.,Wu,Jiazhen.,...&Wang,Hongda.(2014).Studying the Nucleated Mammalian Cell Membrane by Single Molecule Approaches.plos one,9(5),5057-5115. |
| MLA | Zhao, Weidong,et al."Studying the Nucleated Mammalian Cell Membrane by Single Molecule Approaches".plos one 9.5(2014):5057-5115. |
入库方式： OAI收割
来源：长春应用化学研究所
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