Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines
文献类型：期刊论文
| 作者 | Wang, Chao ; Zeng, Zhenyu ; Liu, Qiong ; Zhang,Renli ; Ni,Jiazuan |
| 刊名 | international journal of molecular sciences
 |
| 出版日期 | 2014 |
| 卷号 | 15期号:11页码:21331-21347 |
| 关键词 | STRESS-INDUCED APOPTOSIS GENOME-WIDE ASSOCIATION OXIDATIVE STRESS ALZHEIMERS-DISEASE CLUSTERIN/APOLIPOPROTEIN-J IDENTIFIES VARIANTS DEATH CANCER EXPRESSION PROTEIN |
| 通讯作者 | liu,q |
| 英文摘要 | apoptosis, as a programmed cell death process, is essential for the maintenance of tissue function in organisms. alteration of this process is linked to many diseases. over-expression of clusterin (clu) can antagonize apoptosis in various cells. selenium (se) is an essential trace element for human health. its biological function is also associated with cell apoptosis. to explore the function of clu and the impact of se in the process of apoptosis, several short-hairpin rnas (shrna) were designed for the construction of two sets of recombinant plasmids: one set for plasmid-transfection of mouse neuroblastoma n2a cells (n2a cells); and the other set for lentiviral infection of human neuroblastoma sh-sy5y cells (sh-sy5y cells). these shrnas specifically and efficiently interfered with the intracellular expression of clu at both the mrna and protein levels. the clu-knockdown cells showed apoptosis-related features, including down-regulation of antioxidative capacity and the bcl-2/bax ratio and up-regulation of caspase-8 activity. se-methylselenocysteine (msc) at an optimum concentration of 1 mu m could reverse the alteration in antioxidative capacity, bcl2/bax ratio and caspase-8 activity caused by clu-knockdown, thus inhibiting apoptosis and maintaining cell viability. the results hereby imply the potentiality of clu and se in neuroprotection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345529200116 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57394]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Zeng, Zhenyu,Liu, Qiong,et al. Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines[J]. international journal of molecular sciences,2014,15(11):21331-21347. |
| APA | Wang, Chao,Zeng, Zhenyu,Liu, Qiong,Zhang,Renli,&Ni,Jiazuan.(2014).Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines.international journal of molecular sciences,15(11),21331-21347. |
| MLA | Wang, Chao,et al."Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines".international journal of molecular sciences 15.11(2014):21331-21347. |
入库方式： OAI收割
来源：长春应用化学研究所
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