The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Yang, Dong ; Zhao, Jiadi ; Liu, Shu ; Song,Fengrui ; Liu,Zhiqiang |
| 刊名 | analytical methods
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:10页码:3353-3359 |
| 关键词 | TYPE-2 DIABETES-MELLITUS METABOLOMICS IONIZATION |
| 通讯作者 | liu,s |
| 英文摘要 | alpha-glucosidase inhibitors have been used for the management of type 2 diabetes (t2dm) for a long time. the natural alpha-glucosidase inhibitors from chinese herbal medicine have become an attractive therapeutic approach for treating t2dm due to their low toxicity. in this study, a new kind of in vitro alpha-glucosidase inhibition assay based on using maltose as the substrate was developed, and this new established method was used to determine the alpha-glucosidase inhibitory activities of polygonum multiflorum and four anthraquinone compounds. as a result, polygonum multiflorum showed 50% alpha-glucosidase inhibition at the concentration of 0.0032 mg ml(-1) and four anthraquinone compounds including emodin, aloe-emodin, physcion and rhein showed strong alpha-glucosidase inhibitory activities with ic50 values ranging from 4.12 mu m to 5.68 mu m, respectively. moreover, the centrifugal ultrafiltration with lc-esi-msn was used to screen and identify active alpha-glucosidase inhibitors from polygonum multiflorum extract and nine small-molecule active compounds were successfully identified as potential alpha-glucosidase inhibitors. five compounds belonged to the anthraquinone group including emodin, physcion, aloe-emodin, rhein and physcion-8-o-glucoside, while the others were catechin, resveratrol, 2,3,5,4 '-tetrahydroxystilbene-2-o-glucoside and torachryson-8-o-glucoside. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335224900025 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57475]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang, Dong,Zhao, Jiadi,Liu, Shu,et al. The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry[J]. analytical methods,2014,6(10):3353-3359. |
| APA | Yang, Dong,Zhao, Jiadi,Liu, Shu,Song,Fengrui,&Liu,Zhiqiang.(2014).The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry.analytical methods,6(10),3353-3359. |
| MLA | Yang, Dong,et al."The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry".analytical methods 6.10(2014):3353-3359. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。