Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes
文献类型：期刊论文
| 作者 | Ma, Lina ; Ma, Lan ; Su, Min ; Wang,Zhenxin |
| 刊名 | analytical methods
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:12页码:4366-4372 |
| 关键词 | COLORIMETRIC ASSAY CAPILLARY-ELECTROPHORESIS APTAMER FLUORESCENCE BIOSENSOR AMPLIFICATION AGGREGATION INHIBITION FLUID |
| 通讯作者 | wang,zx |
| 英文摘要 | herein, a gold nanoparticle (gnp)-based colorimetric aptasensor has been developed for detecting adenosine with exonuclease iii (exo iii) assisted recycling amplification. in this aptasensor, two kinds of single-stranded oligonucleotide functionalized gnp probes (oligo 1-gnps and oligo 2-gnps) are firstly hybridized with one complementary single-stranded oligonucleotide (linker) with the motif of adenosine aptamer, resulting in the formation of gnp aggregates (cross-linked gnps). in the presence of adenosine, the linker would form a double-stranded dna (dsdna) with a recessed 3'-terminus due to its structural switching property, which would lead to disassembly of the cross-linked gnps. however, upon the addition of exo iii, the dsdna is enzymatically digested from the 3'-hydroxyl termini, liberating the adenosine. the released adenosine can then interact with another linker and a new round of enzymatic digestion is started. therefore, remarkable signal amplification is achieved because the disassembly of cross-linked gnps is strongly dependent on the free adenosine in the reaction mixture. the experimental results demonstrate that the exo iii-assisted gnp-based colorimetric aptasensor is more sensitive (ca. 10 times) than that without exo iii amplification. the exo iii-assisted gnp-based colorimetric aptasensor detects adenosine downs to 5.6 nm, with a linear relationship from 10 nm to 1 mm. moreover, the exo iii-assisted gnp-based colorimetric aptasensor has been successfully applied to detect jinshuibao capsule (jsbc) containing adenosine in both a buffer solution and a diluted human serum, demonstrating that this assay has great potential to be employed for detecting adenosine in real samples. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-10-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57476]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ma, Lina,Ma, Lan,Su, Min,et al. Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes[J]. analytical methods,2014,6(12):4366-4372. |
| APA | Ma, Lina,Ma, Lan,Su, Min,&Wang,Zhenxin.(2014).Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes.analytical methods,6(12),4366-4372. |
| MLA | Ma, Lina,et al."Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes".analytical methods 6.12(2014):4366-4372. |
入库方式： OAI收割
来源：长春应用化学研究所
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