Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi
文献类型：期刊论文
| 作者 | Wang, Jing ; Liu, Shu ; Li, Sainan ; Song,Fengrui ; Zhang,Yuchi ; Liu,Zhiqiang ; Liu,Chun-ming |
| 刊名 | analytical methods
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:15页码:5918-5924 |
| 关键词 | MASS-SPECTROMETRY FLAVONOIDS HPLC MS PHARMACOKINETICS METABOLOMICS LEAVES |
| 通讯作者 | liu,s |
| 英文摘要 | diabetes mellitus (dm) has become a worldwide health problem alpha-glucosidase inhibitors are widely used in the treatment of type 2 diabetes, and natural products are considered as the promising sources of alpha-glucosidase inhibitors. in this research, ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization tandem mass spectrometry (ultrafiltration lc-pda-esi/ms) were applied to screen and identify alpha-glucosidase inhibitors from scutellaria baicalensis. following this, reverse-phase medium-pressure liquid chromatography (rp-mplc) was applied to separate and isolate the active constituents. as a result, eleven major compounds in s. baicalensis were screened and identified as alpha-glucosidase inhibitors by ultrafiltration lc-pda-esi/ms and six ligands, baicalin, wogonoside, 5,7,3,2',6'-pentahydroxy flavanone, chrysin-6-c-arabinosyl-8-c-glucoside, chrysin-6-c-glucosyl-8-c-arabinoside and wogonin, were isolated by rp-mplc. the purities of the six compounds were 89.7%, 83.5%, 76.2%, 93.7%, 75.1% and 96.2%, respectively, as determined by high performance liquid chromatography. the results demonstrate that the method for systematic isolation of bioactive components in s. baicalensis guided by ultrafiltration lc-pda-esi/ms is a feasible and efficient technique and could be expected to extend for the separation of other enzyme inhibitors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-10-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57478]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Liu, Shu,Li, Sainan,et al. Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi[J]. analytical methods,2014,6(15):5918-5924. |
| APA | Wang, Jing.,Liu, Shu.,Li, Sainan.,Song,Fengrui.,Zhang,Yuchi.,...&Liu,Chun-ming.(2014).Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi.analytical methods,6(15),5918-5924. |
| MLA | Wang, Jing,et al."Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi".analytical methods 6.15(2014):5918-5924. |
入库方式： OAI收割
来源：长春应用化学研究所
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