A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin
文献类型：期刊论文
| 作者 | Wang, Fangyuan ; Li, Yongxin ; Li, Wenying ; Zhang,Qingfeng ; Chen,Jian ; Zhou,Huipeng ; Yu,Cong |
| 刊名 | analytical methods
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:15页码:6105-6109 |
| 关键词 | ASCORBIC-ACID 2-PHOSPHATE ALAMAR BLUE ASSAY GOLD NANOPARTICLES PERYLENE-PROBE RESAZURIN TIME PYROPHOSPHATE TETRAPHENYLETHYLENE CYTOTOXICITY AGGREGATION |
| 通讯作者 | yu,c |
| 英文摘要 | in the present study, a facile fluorescence turn-on approach for the detection of alkaline phosphatase (alp) activity has been developed. l-ascorbic acid-2-phosphate (aap), a substrate of alp, could be hydrolyzed by alp to give l-ascorbic acid. l-ascorbic acid then reduced resazurin to resorufin, which resulted in a turn-on fluorescence signal. the fluorescence intensity increase could be directly related to the amount of alp added to the assay solution. the assay is very sensitive, as 0.12 mu ml(-1) alp could be clearly detected. sodium orthovanadate (na3vo4), a well-known alp inhibitor, was tested, and a clear inhibition effect was observed. the results suggest that our method could be used for alp activity sensing related various biochemical applications and for the screening of alp inhibitors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339468600091 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57479]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Fangyuan,Li, Yongxin,Li, Wenying,et al. A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin[J]. analytical methods,2014,6(15):6105-6109. |
| APA | Wang, Fangyuan.,Li, Yongxin.,Li, Wenying.,Zhang,Qingfeng.,Chen,Jian.,...&Yu,Cong.(2014).A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin.analytical methods,6(15),6105-6109. |
| MLA | Wang, Fangyuan,et al."A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin".analytical methods 6.15(2014):6105-6109. |
入库方式： OAI收割
来源：长春应用化学研究所
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