Studying chemical-regulation of intracellular kinase activity by peptide microarray-based assay with gold nanoparticle probes
文献类型：期刊论文
| 作者 | Li, Tao ; Su, Min ; Ma, Lan ; Liu,Dianjun ; Wang,Zhenxin |
| 刊名 | analytical methods
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:23页码:9404-9409 |
| 关键词 | DEPENDENT PROTEIN-KINASE CAMP/PROTEIN KINASE SIGNALING PATHWAY MASS-SPECTROMETRY INHIBITORS CELLS CANCER PKA PHOSPHORYLATION QUANTIFICATION |
| 通讯作者 | wang,zx |
| 英文摘要 | in the present work, the chemical regulation of intracellular kinase activity has been studied by a peptide microarray-based resonance light scattering (rls) assay with gold nanoparticle (aunp) probes. after interactions of five cell lines and seven compounds (six potential inhibitors of cyclic adenosine monophosphate (camp)-dependent protein kinase a (pka) and forskolin (fsk)), we found that the intracellular pka activity is strongly regulated by the extracellular compounds and that the compounds affect the intracellular pka activity in a dose-dependent manner. the experimental results demonstrate that the peptide microarray-based rls assay can be employed to quantitatively determine the efficiency of the inhibitor/activator on the kinase activity at the cellular level. in addition, chemical-mediated fluctuation of the pka activity in different cell phases (e.g., g2/m phase and m/g1 phase) was successfully detected, which also demonstrates the utility of the approach for monitoring chemical regulation of intracellular kinase activity. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344964700029 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/57483]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, Tao,Su, Min,Ma, Lan,et al. Studying chemical-regulation of intracellular kinase activity by peptide microarray-based assay with gold nanoparticle probes[J]. analytical methods,2014,6(23):9404-9409. |
| APA | Li, Tao,Su, Min,Ma, Lan,Liu,Dianjun,&Wang,Zhenxin.(2014).Studying chemical-regulation of intracellular kinase activity by peptide microarray-based assay with gold nanoparticle probes.analytical methods,6(23),9404-9409. |
| MLA | Li, Tao,et al."Studying chemical-regulation of intracellular kinase activity by peptide microarray-based assay with gold nanoparticle probes".analytical methods 6.23(2014):9404-9409. |
入库方式： OAI收割
来源：长春应用化学研究所
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