Spectroscopic and molecular modeling study of cyanine dye interacting with human serum transferrin
文献类型：期刊论文
| 作者 | Zhang, Xiu-feng1; Chen, Lei1; Yang, Qian-fan2; Li, Qian2; Sun, Xiao-ran1; Chen, Hong-bo1; Yang, Guang1; Tang, Ya-lin2 |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2015-03-20 |
| 卷号 | 469页码:187-193 |
| 关键词 | Transferrin Cyanine dye Fluorescent quenching Conformational transition CD Docking |
| 英文摘要 | The study of binding mechanisms and any associated conformational changes of human serum transferrin (Tf) on interaction with cyanine dye 3,3'-di(3-sulfopropy1)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylam-monium salt (MTC) are of great importance in the process of Tf targeting dye delivery into cancer cells. It is possible to lay theoretical foundations for cyanine dye as a potential photosensitizer to achieve the photodynamic therapy (PDT). The mechanisms of interaction between MTC and Tf were portrayed by means of fluorescence spectra, UV-vis absorption spectra, synchronous fluorescence spectra, circular dichroism (CD) and molecular dynamic docking. The data of fluorescence spectra displayed that the formation of MTC-Tf complex is a static quenching process through van der Waals forces and hydrogen bonds with a high affinity of 10(9) M-1. Binding distance between MTC and Tf substantiated that the non-radioactive energy transfer mechanism is also involved in the fluorescence quenching of protein. Furthermore, structural analysis indicated that MTC binding result in an increased of ce.-helix content and an increased hydrophobic around the tryptophan residues of Tf as well as a certain structural changes in Tf, which confirmed by the CD, synchronous fluorescence and UV-vis experiments. Additionally, the results of molecular dynamic docking elucidated that the dye was located in N-lobe of Tf. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-02 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28205]  |
| 专题 | 化学研究所_分子动态与稳态结构实验室 |
| 作者单位 | 1.Hebei United Univ, Coll Chem Engn, Tangshan 063009, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiu-feng,Chen, Lei,Yang, Qian-fan,et al. Spectroscopic and molecular modeling study of cyanine dye interacting with human serum transferrin[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2015,469:187-193. |
| APA | Zhang, Xiu-feng.,Chen, Lei.,Yang, Qian-fan.,Li, Qian.,Sun, Xiao-ran.,...&Tang, Ya-lin.(2015).Spectroscopic and molecular modeling study of cyanine dye interacting with human serum transferrin.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,469,187-193. |
| MLA | Zhang, Xiu-feng,et al."Spectroscopic and molecular modeling study of cyanine dye interacting with human serum transferrin".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 469(2015):187-193. |
入库方式： OAI收割
来源：化学研究所
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