Mesenchymal stem cells attenuated PLGA-induced inflammatory responses by inhibiting host DC maturation and function
文献类型：期刊论文
| 作者 | Zhu, Heng1; Yang, Fei2; Tang, Bo1; Li, Xi-Mei1; Chu, Ya-Nan1; Liu, Yuan-Lin1; Wang, Shen-Guo2; Wu, De-Cheng2; Zhang, Yi1 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 53页码:688-698 |
| 关键词 | Poly (lactide-co-glycolide) Dendritic cells Mesenchymal stem cells Host inflammatory responses |
| 英文摘要 | The poly lactic-co-glycolic acid (PLGA) bio-scaffold is a biodegradable scaffold commonly used for tissue repair. However, implanted PLGA scaffolds usually cause serious inflammatory responses around grafts. To improve PLGA scaffold-based tissue repair, it is important to control the PLGA-mediated inflammatory responses. Recent evidence indicated that PLGA induce dendritic cell (DC) maturation in vitro, which may initiate host immune responses; In the present study, we explored the modulatory effects of mesenchymal stem cells (MSC) on PLGA-induced DCs (PLGA-DC). We found that mouse MSCs inhibited PLGA-DC dendrite formation, as well as co-stimulatory molecule and pro-inflammatory factor expression. Functionally, MSC-educated PLGA-DCs promoted Th2 and regulatory T cell differentiation but suppressed Th1 and Th17 cell differentiation. Mechanistically, we determined that PLGA elicited DC maturation via inducing phosphorylation of p38/MAPK and ERK/MAPK pathway proteins in DCs. Moreover, MSCs suppressed PLGA-DCs by partially inactivating those pathways. Most importantly, we found that the MSCs were capable of suppressing DC maturation and immune function in vivo. Also, the proportion of mature DCs in the mice that received MSC-PLGA constructs greatly decreased compared with that of their PLGA-film implantation counterparts. Additionally, MSCs co-delivery increased regulatory T and Th2 cells but decreased the Th1 and Th17 cell numbers in the host spleens. Histological analysis showed that MSCs alleviated the inflammatory responses around the grafted PLGA scaffolds. In summary, our findings reveal a novel function for MSCs in suppressing PLGA-induced host inflammatory response and suggest that DCs are a new cellular target in improving PLGA scaffold-based tissue repair. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-02 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28020]  |
| 专题 | 化学研究所_高分子物理与化学实验室 |
| 作者单位 | 1.Inst Basic Med Sci, Dept Cell Biol, Beijing 100850, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, BNLMS, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Heng,Yang, Fei,Tang, Bo,et al. Mesenchymal stem cells attenuated PLGA-induced inflammatory responses by inhibiting host DC maturation and function[J]. BIOMATERIALS,2015,53:688-698. |
| APA | Zhu, Heng.,Yang, Fei.,Tang, Bo.,Li, Xi-Mei.,Chu, Ya-Nan.,...&Zhang, Yi.(2015).Mesenchymal stem cells attenuated PLGA-induced inflammatory responses by inhibiting host DC maturation and function.BIOMATERIALS,53,688-698. |
| MLA | Zhu, Heng,et al."Mesenchymal stem cells attenuated PLGA-induced inflammatory responses by inhibiting host DC maturation and function".BIOMATERIALS 53(2015):688-698. |
入库方式： OAI收割
来源：化学研究所
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