Development of Large-Scale Size-Controlled Adult Pancreatic Progenitor Cell Clusters by an Inkjet-Printing Technique
文献类型：期刊论文
| 作者 | Yang, Jia1; Zhou, Fang1; Xing, Rubo1; Lin, Yuan1; Han, Yanchun1; Teng, Chunbo2; Wang, Qian3 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015-06-03 |
| 卷号 | 7期号:21页码:11624-11630 |
| 关键词 | tobacco mosaic virus inkjet printing surface pattern pancreatic progenitor cell cell cluster |
| 英文摘要 | The generation of transplantable beta-cells from pancreatic progenitor cells (PPCs) could serve as an ideal cell-based therapy for diabetes. Because the transplant efficiency depends on the size of islet-like clusters, it becomes one of the key research topics to produce PPCs with controlled cluster sizes in a scalable manner. In this study, we used inkjet printing to pattern biogenic nanoparticles, i.e., mutant tobacco mosaic virus (TMV), with different spot sizes to support the formation of multicellular clusters by PPCs. We successfully achieved TMV particle patterns with variable features and sizes by adjusting the surface wettability and printing speed. The spot sizes of cell-adhesive TMV mutant arrays were in the range of 50-150 mu m diameter. Mouse PPCs were seeded on the TMV-RGD (arginine-glycine-aspartate)-patterned polystyrene (PS) substrate, which consists of areas that either favor (TMV-RGD) or prohibit (bare PS) cell adhesion. The PPCs stably attached, proliferated on top of the TMV-RGD support, thus resulting in the formation of uniform and confluent PPC clusters. Furthermore, the aggregated PPCs also maintained their multipotency and were positive for E-cadherin, indicating that the formation of cell-cell junctions is critical for enhanced cell-cell contact. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-02 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28198]  |
| 专题 | 化学研究所_高分子物理与化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China 3.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA |
| 推荐引用方式 GB/T 7714 | Yang, Jia,Zhou, Fang,Xing, Rubo,et al. Development of Large-Scale Size-Controlled Adult Pancreatic Progenitor Cell Clusters by an Inkjet-Printing Technique[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(21):11624-11630. |
| APA | Yang, Jia.,Zhou, Fang.,Xing, Rubo.,Lin, Yuan.,Han, Yanchun.,...&Wang, Qian.(2015).Development of Large-Scale Size-Controlled Adult Pancreatic Progenitor Cell Clusters by an Inkjet-Printing Technique.ACS APPLIED MATERIALS & INTERFACES,7(21),11624-11630. |
| MLA | Yang, Jia,et al."Development of Large-Scale Size-Controlled Adult Pancreatic Progenitor Cell Clusters by an Inkjet-Printing Technique".ACS APPLIED MATERIALS & INTERFACES 7.21(2015):11624-11630. |
入库方式： OAI收割
来源：化学研究所
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