Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA
文献类型：期刊论文
| 作者 | Guan, Lingmei1; Huang, Saipeng2; Chen, Zhao3; Li, Yanchao2; Liu, Ke1; Liu, Yang2; Du, Libo2 |
| 刊名 | JOURNAL OF NANOPARTICLE RESEARCH
 |
| 出版日期 | 2015-09-25 |
| 卷号 | 17期号:9 |
| 关键词 | Gene carriers Nanoparticles PAMAM siRNA delivery Fluorescence Drug delivery |
| 英文摘要 | Visual detection of gene vectors has attracted a great deal of attention due to the application of these vectors in monitoring and evaluating the effect of gene carriers in living cells. A non-viral vector, the fluorescent PAMAM dendrimer (F-PAMAM), was synthesized through conjugation of PAMAM dendrimers and fluorescein. In vitro and ex vivo experiments show that F-PAMAM exhibits superphotostability, low cytotoxicity and facilitates endocytosis by A549 cells. The vector has a high siRNA binding affinity and it increases the efficiency of cy5-siRNA delivery in A549 cells, in comparison with a cy5-siRNA monomer. Our results provide a new method for simultaneously monitoring the delivery of siRNA and its non-viral carriers in living cells. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-03 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28428]  |
| 专题 | 化学研究所_分子动态与稳态结构实验室 |
| 作者单位 | 1.Sichuan Univ, State Key Lab Bioresources & Ecoenvironm, Minist Educ, Coll Life Sci, Chengdu 610064, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Mol Sci, Beijing 100190, Peoples R China 3.Xi An Jiao Tong Univ, Sch Sci, Xian 710049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Lingmei,Huang, Saipeng,Chen, Zhao,et al. Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA[J]. JOURNAL OF NANOPARTICLE RESEARCH,2015,17(9). |
| APA | Guan, Lingmei.,Huang, Saipeng.,Chen, Zhao.,Li, Yanchao.,Liu, Ke.,...&Du, Libo.(2015).Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA.JOURNAL OF NANOPARTICLE RESEARCH,17(9). |
| MLA | Guan, Lingmei,et al."Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA".JOURNAL OF NANOPARTICLE RESEARCH 17.9(2015). |
入库方式： OAI收割
来源：化学研究所
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