MERCURIC CHLORIDE DAMAGES THE THYLAKOID MEMBRANE STRUCTURE OF CHLOROPLASTS AND ALTERS THE PROFILES OF SOD, CAT AND EST ISOZYMES IN Chlamydomonas reinhardtii
文献类型:期刊论文
作者 | Han, Yong-Guang1,2; Tan, Qiu-Ju1; Liu, Ke1; Liu, Yang3; Du, Lin-Fang1 |
刊名 | FRESENIUS ENVIRONMENTAL BULLETIN
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出版日期 | 2015 |
卷号 | 24期号:2A页码:676-682 |
关键词 | mercuric chloride Chlamydomonas reinhardtii fluorescence spectroscopy reactive oxygen species isoenzymes |
英文摘要 | The effect of mercuric chloride on the thylakoid membrane structure and the banding patterns of antioxidant enzymes in Chlamydomonas reinhardtii (C. reinhardtii) were explored. Three strains of C. reinhardtii, CC-124 (wild type), CC-2473 (psbA) and CC-2913 (NL-11) were used. Ours results showed that Hg2+ inhibited cell growth and reduced the chlorophyll content, strain CC-2913 being the most sensitive to Hg2+. The maximum fluorescence emission peak at an excitation wavelength of 278 or 295nm generally declined in response to increasing Hg2+ concentration indicating that Hg2+ seriously damaged the protein structure. The isoenzymic patterns of several antioxidant enzymes, SOD, CAT and EST were assayed, but the isoenzymic patterns differed between strains, indicating that each strain of alga dealt with Hg2+-induced stress differently and that the mechanism involved might be complex. It is also speculated that CAT might play an important protective role during Hg2+-induced stress. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2015-11-03 |
源URL | [http://ir.iccas.ac.cn/handle/121111/28441] ![]() |
专题 | 化学研究所_分子动态与稳态结构实验室 |
作者单位 | 1.Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Sichuan, Peoples R China 2.Henan Univ Tradit Chinese Med, Zhengzhou 45008, Henan, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Han, Yong-Guang,Tan, Qiu-Ju,Liu, Ke,et al. MERCURIC CHLORIDE DAMAGES THE THYLAKOID MEMBRANE STRUCTURE OF CHLOROPLASTS AND ALTERS THE PROFILES OF SOD, CAT AND EST ISOZYMES IN Chlamydomonas reinhardtii[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2015,24(2A):676-682. |
APA | Han, Yong-Guang,Tan, Qiu-Ju,Liu, Ke,Liu, Yang,&Du, Lin-Fang.(2015).MERCURIC CHLORIDE DAMAGES THE THYLAKOID MEMBRANE STRUCTURE OF CHLOROPLASTS AND ALTERS THE PROFILES OF SOD, CAT AND EST ISOZYMES IN Chlamydomonas reinhardtii.FRESENIUS ENVIRONMENTAL BULLETIN,24(2A),676-682. |
MLA | Han, Yong-Guang,et al."MERCURIC CHLORIDE DAMAGES THE THYLAKOID MEMBRANE STRUCTURE OF CHLOROPLASTS AND ALTERS THE PROFILES OF SOD, CAT AND EST ISOZYMES IN Chlamydomonas reinhardtii".FRESENIUS ENVIRONMENTAL BULLETIN 24.2A(2015):676-682. |
入库方式: OAI收割
来源:化学研究所
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