Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy
文献类型：期刊论文
| 作者 | Liu, Jianli1; Zhang, Xuejie1; Yang, Hang2; Yuan, Jinghe1; Wei, Hongping2; Yu, Junping2; Fang, Xiaohong1 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:37页码:15245-15250 |
| 英文摘要 | The cell wall binding domain (CBD) of bacteriophage lysins can recognize target bacteria with extraordinary specificity through binding to bacterial peptidoglycan, thus it is a promising new probe to identify the corresponding bacterial pathogen. In this work, we used atomic force microscopy (AFM) based single-molecule force spectroscopy to investigate the interaction between the CBD of lysin PlyV12 (PlyV12C) and pathogenic bacterium Staphylococcus aureus (S. aureus). The binding forces of PlyV12C with S. aureus have been measured, and the dissociation process of their binding complex has been characterized. Furthermore, we compared the interactions of PlyV12C-S. aureus and antibody-S. aureus. It is revealed that PlyV12C has a comparable affinity to bacterial peptidoglycans as that of the S. aureus antibody. The results provide new information on the binding properties of lysin CBD with bacterium, and the application of lysin CBD in bacterium detection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-03 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28407]  |
| 专题 | 化学研究所_分子纳米结构与纳米技术实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jianli,Zhang, Xuejie,Yang, Hang,et al. Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy[J]. NANOSCALE,2015,7(37):15245-15250. |
| APA | Liu, Jianli.,Zhang, Xuejie.,Yang, Hang.,Yuan, Jinghe.,Wei, Hongping.,...&Fang, Xiaohong.(2015).Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy.NANOSCALE,7(37),15245-15250. |
| MLA | Liu, Jianli,et al."Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy".NANOSCALE 7.37(2015):15245-15250. |
入库方式： OAI收割
来源：化学研究所
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