Responsive Polypeptide-DNA Hydrogel Crosslinked by G-quadruplex
文献类型：期刊论文
| 作者 | Shao Yu1; Li Chuang1; Zhou Xu1; Chen Ping2; Yang Zhongqiang1; Li Zhibo2; Liu Dongsheng1 |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2015-08-15 |
| 卷号 | 73期号:8页码:815-818 |
| 关键词 | DNA polypeptide self-assembly G-quadruplex hydrogel responsiveness |
| 英文摘要 | In the past thirty years, DNA molecule has received considerable attention as a promising building material due to its precise base-paring recognition, the programmable sequences, the predictable secondary structures, etc. Based on DNA self-assembly technique, DNA supramolecular hydrogels can be fabricated which are crosslinked networks swollen in an aqueous phase by the supramolecular interaction and also have widely applications in the biomedical field. In this paper, a new type of DNA-based supramolecular hydrogel was prepared by simply mixing two components: Polypeptide-DNA and G-quadruplex. We have successfully synthesized single-stranded DNA (ssDNA) grafted polypeptide via the copper-catalyzed click reaction. By introducing the intermolecular G-quadruplex structure, which is rich in guanine and is capable of forming a four-stranded structure in the presence of potassium, the hydrogel can be formed by DNA hybridization in a fast and in-situ manner and also obtain thermal and ionic responsive gel-sol transition, respectively. In this experiment, we firstly used polyacrylamide gel electrophoresis (PAGE), circular dichroism spectra (CD), UV-Vis spectroscopy to demonstrate the G-quadruplex structure formation, and the assembling process was efficient which are formed the parallel structure and the melting temperature (T-m) was about 43 degrees C. Then by the rheological tests, the shear-storage modulus (G') was obviously higher (up to 2.4 kPa) than the shear-loss modulus (G '') over the entire time range, and a minimum concentration of 0.5 wt% polypeptide-DNA was required to form a gel-like state, and the mechanical strength of the DNA hydrogels increased with increasing concentration of the DNA building blocks. What's more, the hydrogels can switch between the gel and the sol state when changing the temperature from 25 to 50 degrees C many times, indicating that the DNA hydrogels respond to thermal in a reversible way. At last, by using the properties of crown ether complexing potassium ion, the DNA hydrogel can obtain the ion responsiveness properties. Due to its designable responsiveness and good biocompatibility, this supramolecular hydrogel would have great potential applications in tissue engineering and 3D bioprinting field. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-03 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28422]  |
| 专题 | 化学研究所_高分子物理与化学实验室 |
| 作者单位 | 1.Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao Yu,Li Chuang,Zhou Xu,et al. Responsive Polypeptide-DNA Hydrogel Crosslinked by G-quadruplex[J]. ACTA CHIMICA SINICA,2015,73(8):815-818. |
| APA | Shao Yu.,Li Chuang.,Zhou Xu.,Chen Ping.,Yang Zhongqiang.,...&Liu Dongsheng.(2015).Responsive Polypeptide-DNA Hydrogel Crosslinked by G-quadruplex.ACTA CHIMICA SINICA,73(8),815-818. |
| MLA | Shao Yu,et al."Responsive Polypeptide-DNA Hydrogel Crosslinked by G-quadruplex".ACTA CHIMICA SINICA 73.8(2015):815-818. |
入库方式： OAI收割
来源：化学研究所
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