Probing the Adjustments of Macromolecules during Their Surface Adsorption
文献类型：期刊论文
| 作者 | Jia, Pengxiang1; He, Min1; Gong, Yongkuan1; Chu, Xiao1,2; Yang, Jingfa2; Zhao, Jiang2 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 7期号:12页码:6422-6429 |
| 关键词 | protein adsorption QCM-D SPR conformation polymer brushes |
| 英文摘要 | Thiol-terminated polymers poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC-SH), poly(N,N-isopropylacrylamide) (PNIPAM-SH), and poly(tert-butyl acrylate) (PtBA-SH) were synthesized, and the polymers were grafted on the gold surfaces of quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR) sensor chips to form brushes. The grafting process of the polymer brushes as well as protein adsorption onto the brush layers was monitored by in situ QCM-D and SPR techniques. By examining the changes in frequency and dissipation factor as well as the value of partial derivative D/partial derivative f from QCM-D measurements, different stages of the polymer grafting and protein adsorption are distinguished. The most interesting discovery is the conformation change of BSA protein adsorption from a weakly adsorbed native state to a strongly immobilized denatured state on the polymer brushes. The corresponding change in BSA adsorption from a reversible state to an irreversible state was confirmed by SPR measurements. The adsorption of protein on the polymer brushes' surface relies largely on interaction between the protein and the polymers, and the stronger hydrophilicity of the surfaces is proved to be more effective to suppress the protein adsorption. Analysis of the D-f plot of QCM-D measurements helps to characterize different binding strength of protein and the underlying polymer surface. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-11-03 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/28521]  |
| 专题 | 化学研究所_高分子物理与化学实验室 |
| 作者单位 | 1.NW Univ Xian, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710069, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Pengxiang,He, Min,Gong, Yongkuan,et al. Probing the Adjustments of Macromolecules during Their Surface Adsorption[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(12):6422-6429. |
| APA | Jia, Pengxiang,He, Min,Gong, Yongkuan,Chu, Xiao,Yang, Jingfa,&Zhao, Jiang.(2015).Probing the Adjustments of Macromolecules during Their Surface Adsorption.ACS APPLIED MATERIALS & INTERFACES,7(12),6422-6429. |
| MLA | Jia, Pengxiang,et al."Probing the Adjustments of Macromolecules during Their Surface Adsorption".ACS APPLIED MATERIALS & INTERFACES 7.12(2015):6422-6429. |
入库方式： OAI收割
来源：化学研究所
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