Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis
文献类型：期刊论文
| 作者 | Liu Jing1,2; Wang Fang-Jun1; Zhang Zhen-Bin1,2; Zou Han-Fa1 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 41期号:1页码:10-14 |
| 关键词 | Reversed phase polymer-based monolithic column Enzyme reactor Liquid chromatography coupled with mass spectrometry |
| 英文摘要 | we developed a reversed phase monolithic column based enzyme reactor for protein analysis. using the number of identified unique peptides, sequence coverage and the level of missed cleavage as the evaluation indicators, we systematically investigated the influence of buffer solution, porous structure of the column, digestion time and hydrophobicity of solid phase on the digestion performance of enzyme reactor. it had demonstrated that the reversed phase monolithic column based enzyme reactor had high digestion efficiency and might be further extended to the proteome analysis of complex samples. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | digestion ; identification ; proteolysis ; system |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315021700002 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137471]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Jing,Wang Fang-Jun,Zhang Zhen-Bin,et al. Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis[J]. chinese journal of analytical chemistry,2013,41(1):10-14. |
| APA | Liu Jing,Wang Fang-Jun,Zhang Zhen-Bin,&Zou Han-Fa.(2013).Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis.chinese journal of analytical chemistry,41(1),10-14. |
| MLA | Liu Jing,et al."Reversed Phase Monolithic Column Based Enzyme Reactor for Protein Analysis".chinese journal of analytical chemistry 41.1(2013):10-14. |
入库方式： OAI收割
来源：大连化学物理研究所
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