Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways
文献类型：期刊论文
| 作者 | Wang, Fangjun1,2; Blanchard, Alexandre P.2,3; Elisma, Fred2; Granger, Matthew2,3; Xu, Hongbin2,3; Bennett, Steffany A. L.2,3; Figeys, Daniel2; Zou, Hanfa1 |
| 刊名 | proteomics
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 13期号:8页码:1292-1305 |
| 关键词 | Alzheimer's disease Animal model Animal proteomics MS Phosphoproteomics TgCRND8 |
| 英文摘要 | sustained exposure to soluble amyloid (a42) oligomers is predicted to impair synaptic function in the hippocampal-entorhinal circuit, signaling synaptic loss and precipitating cognitive impairment in alzheimer's disease. regional changes in overall patterns of protein phosphorylation are likely crucial to promote transition from a presymptomatic to a symptomatic state in response to accumulating a42. here, we used unbiased proteomic approaches to compare the phosphoproteome of presymptomatic and symptomatic tgcrnd8 mice and identify network disruptions in signaling pathways implicated in the manifestation of behavioral indices of learning and memory impairment. phosphopeptide enrichment with triple isotopic dimethylation labeling combined with online multidimensional separation and ms was used to profile phosphoproteome changes in 2- and 6-month-old tgcrnd8 mice and congenic littermate controls. we identified 1026 phosphopeptides representing 1168 phosphorylation sites from 476 unique proteins. of these, 595 phosphopeptides from 293 unique proteins were reliably quantified and 139 phosphopeptides were found to change significantly in the hippocampus of tgcrnd8 mice following conversion from a presymptomatic to a symptomatic state. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | response mediator proteins ; amyloid-beta-protein ; myelin basic-protein ; in-vivo ; quantitative proteomics ; tau ; phosphorylation ; aggregation ; binding ; mice |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317684800007 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137475]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China 2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada 3.Univ Ottawa, Neural Regenerat Lab, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Blanchard, Alexandre P.,Elisma, Fred,et al. Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways[J]. proteomics,2013,13(8):1292-1305. |
| APA | Wang, Fangjun.,Blanchard, Alexandre P..,Elisma, Fred.,Granger, Matthew.,Xu, Hongbin.,...&Zou, Hanfa.(2013).Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways.proteomics,13(8),1292-1305. |
| MLA | Wang, Fangjun,et al."Phosphoproteome analysis of an early onset mouse model (TgCRND8) of Alzheimer's disease reveals temporal changes in neuronal and glia signaling pathways".proteomics 13.8(2013):1292-1305. |
入库方式： OAI收割
来源：大连化学物理研究所
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