Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite
文献类型：期刊论文
| 作者 | Xiang, Cuili1; Zou, Yongjin1; Qiu, Shujun1; Sun, Lixian1,2; Xu, Fen1,3; Zhou, Huaiying1 |
| 刊名 | talanta
 |
| 出版日期 | 2013-06-15 |
| 卷号 | 110页码:96-100 |
| 关键词 | Cytochrome c Gold nanoparticles Polyaniline nanoshpere Direct electron transfer Glucose biosensor |
| 英文摘要 | gold nanoparticles (gnps) assembled polyaniline nanospheres (pans) composite were prepared by noncovalent strategy. cytochrome c (cyt c) was electrostatically adsorbed on the gnps/pans modified electrode. the direct electron transfer (det) between cyt c and gnps/pans modified electrode was investigated. cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry (cv) peaks with a formal potential (e-0) of -0.27 v (versus ag/agcl) in ph 7.0 phosphate buffer solution (pbs). the cyt c/gnps/pans modified electrode gives an improved electrocatalytic activity toward the reduction of hydrogen peroxide (h2o2). the catalysis currents increase linearly to the h2o2 concentration in a wide range of 0.01-2.4 mm with a correlation coefficient 0.998. after immobilized glucose oxidase (god) on the cyt c/gnps/pans modified electrode by nafion polymer, a bienzymatic glucose biosensor was further prepared for sensitive detection of glucose. the amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.2 v versus ag/agcl to reduce the enzymatically produced h2o2 with minimal interference from the coexisting electroactive compounds. the proposed glucose biosensor exhibits good response performance to glucose with a wide linear range from 0.01 to 3.2 mm with a correlation coefficient of 0.997. the electrode has high sensitivity of 63.1 [mu a mm(-1) cm(-2) and a detection limit 0.01 mm at the signal-to-noise ratio of 3. moreover, the glucose biosensor possesses good stability and reproducibility. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | glassy-carbon electrode ; platinum nanoparticles ; hydrogen-peroxide ; chitosan ; film ; electrocatalysis ; nanotubes ; proteins ; immobilization ; oxidase |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319089500015 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137500]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Guilin Univ Elect Technol, Guangxi Key Lab Informat Mat, Guilin 541004, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Liaoning Normal Univ, Dept Chem & Chem Engn, Dalian 116029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Cuili,Zou, Yongjin,Qiu, Shujun,et al. Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite[J]. talanta,2013,110:96-100. |
| APA | Xiang, Cuili,Zou, Yongjin,Qiu, Shujun,Sun, Lixian,Xu, Fen,&Zhou, Huaiying.(2013).Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite.talanta,110,96-100. |
| MLA | Xiang, Cuili,et al."Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite".talanta 110(2013):96-100. |
入库方式： OAI收割
来源：大连化学物理研究所
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