Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography
文献类型：期刊论文
| 作者 | Zhang, Zhenbin1,2; Wang, Fangjun1; Ou, Junjie1; Lin, Hui1,2; Dong, Jing1; Zou, Hanfa1 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 405期号:7页码:2265-2271 |
| 关键词 | One pot Sol-gel Butyl-silica hybrid monolith Capillary liquid chromatography Bioseparation |
| 英文摘要 | a butyl-silica hybrid monolithic column for bioseparation by capillary liquid chromatography (clc) was prepared with butyl methacrylate and alkoxysilanes through a "one-pot" process. the effects of polycondensation temperature, volume percentage of n,n'-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by clc with gradient elution. in addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by clc coupled with tandem mass spectrometry. the results demonstrate its potential application in separation of complex biological samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | separation ; electrochromatography ; proteins ; flow |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315043800016 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137531]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,et al. Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography[J]. analytical and bioanalytical chemistry,2013,405(7):2265-2271. |
| APA | Zhang, Zhenbin,Wang, Fangjun,Ou, Junjie,Lin, Hui,Dong, Jing,&Zou, Hanfa.(2013).Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography.analytical and bioanalytical chemistry,405(7),2265-2271. |
| MLA | Zhang, Zhenbin,et al."Preparation of a butyl-silica hybrid monolithic column with a "one-pot" process for bioseparation by capillary liquid chromatography".analytical and bioanalytical chemistry 405.7(2013):2265-2271. |
入库方式： OAI收割
来源：大连化学物理研究所
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