Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria
文献类型：期刊论文
| 作者 | Fu WanTao1,4,5; Zhang JuLin1,6; Zheng ChangBo7; Liu Jing1; An ZhongFu1; Liu HongWen8; Zhang Wei2,3,4 |
| 刊名 | chinese science bulletin
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 58期号:7页码:766-776 |
| 关键词 | marine sponge Hymeniacidon perleve 14-3-3 mRNA RT-PCR infectious bacteria |
| 英文摘要 | sponges (phylum porifera) are the phylogenetically oldest metazoa and highly efficient filter feeders. in the marine ecosystem, they are unconditionally exposed to environmental stresses. understanding the sponge-bacteria interaction is hence of both ecological and biological significance. this study investigated the specific interaction between the sponge hymeniacidon perleve and the non-infectious bacteria, escherichia coli and infectious bacteria, vibrio spp. by measuring the 14-3-3 mrna expression of h. perleve. three partial cdnas of 14-3-3 proteins and partial 18s rna in h. perleve were cloned and sequenced. using reverse-transcription real-time pcr, the 14-3-3 mrna expression of h. perleve was examined when exposed to three common bacteria in aquatic water-e. coli and two vibrio spp. for different time and dosages. h. perleve could efficiently remove e. coli from the water column without self-infection; however vibrio at higher dosages infected h. perleve. when h. perleve was exposed to e. coli (1.1-10(7) cfu ml(-1)), v. anguillarum ii (1.2-10(6) cfu ml(-1)) and v. alginolyticus (3.6-10(5) cfu ml(-1)) for 6 h, the 14-3-3 mrna expression in the v. anguillarum ii and v. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. the 14-3-3 mrna expression in the e. coli group was not significantly different. however, no clear trend was observed on the 14-3-3 transcript levels of h. perleve in response to different doses of v. anguillarum ii for different time. the results demonstrated that infectious bacteria can be discriminated by 14-3-3 mrna expression of sponge h. perleve. |
| WOS标题词 | science & technology |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | activated protein-kinase ; innate immune defense ; suberites-domuncula ; aquatic environments ; induction ; evolution ; apoptosis ; food ; 14-3-3-proteins ; specificity |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315446200010 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137605]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Ocean Univ, Inst Marine Sci Technol & Environm, Dalian 116023, Peoples R China 2.Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioprod Dev, Adelaide, SA 5042, Australia 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia 4.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 5.Key Lab Nearshore Marine Environm Sci & Technol L, Dalian 116023, Peoples R China 6.Dalian Ocean Univ, Grad Sch, Dalian 116023, Peoples R China 7.Dalian Ocean Univ, Vocat & Technol Coll, Dalian 116000, Peoples R China 8.Dalian Environm Monitoring Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu WanTao,Zhang JuLin,Zheng ChangBo,et al. Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria[J]. chinese science bulletin,2013,58(7):766-776. |
| APA | Fu WanTao.,Zhang JuLin.,Zheng ChangBo.,Liu Jing.,An ZhongFu.,...&Zhang Wei.(2013).Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria.chinese science bulletin,58(7),766-776. |
| MLA | Fu WanTao,et al."Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria".chinese science bulletin 58.7(2013):766-776. |
入库方式： OAI收割
来源：大连化学物理研究所
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