Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification
文献类型：期刊论文
| 作者 | Long, Zhen; Guo, Zhimou; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao |
| 刊名 | journal of separation science
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 36期号:24页码:3845-3852 |
| 关键词 | Alkaloids Orthogonality Strong cation exchange Symmetrical peaks |
| 英文摘要 | peak tailing and nonalkaloid coelution usually hinder alkaloid purification. in this study, a 2dlc, strong cation exchange (scx) coupled with positively charged rp (pgrp) lc, was developed to overcome these problems. ten compounds including basic and nonbasic compounds were analyzed. nonbasic compounds, which are coeluted with basic compounds on rp or pgrp columns, were weakly retained on the scx column. in addition, a symmetrical peak shape (tailing factors <1.2) of basic compounds can be obtained in the current system. compared to two other 2d systems, the current system provided the highest orthogonality (r-2 = 0.045). furthermore, the scx coupled with pgrp system was applied for alkaloid purification from a traditional chinese medicine. nineteen alkaloids were obtained and one of them was identified as a novel compound. the overall results demonstrate that the proposed system is a powerful tool for alkaloid purification. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | w. t. wang ; mass-spectrometric detection ; basic compounds ; stationary-phase ; capillary-electrophoresis ; sample preparation ; separation ; performance ; hplc ; extraction |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328138200005 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137665]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification[J]. journal of separation science,2013,36(24):3845-3852. |
| APA | Long, Zhen,Guo, Zhimou,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2013).Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification.journal of separation science,36(24),3845-3852. |
| MLA | Long, Zhen,et al."Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification".journal of separation science 36.24(2013):3845-3852. |
入库方式： OAI收割
来源：大连化学物理研究所
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