Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography
文献类型：期刊论文
| 作者 | Shen, Aijin1; Li, Xiuling1; Dong, Xuefang1; Wei, Jie2; Guo, Zhimou1; Liang, Xinmiao1,2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 1314页码:63-69 |
| 关键词 | Hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) Glutathione Thiol-ene Zwitterionic stationary phase Mixed-mode |
| 英文摘要 | as a naturally hydrophilic peptide, glutathione was facilely immobilized onto silica surface to obtain a novel hydrophilic interaction/cation-exchange mixed-mode chromatographic stationary phase (click te-gsh) via copper-free "thiol-ene" click chemistry. the resulting material was characterized by solid state c-13/cp mas nmr and elemental analysis. the measurement of zeta-potential indicated the cation-exchange characteristics and adjustable surface charge density of click te-gsh material. the influence of acetonitrile content and ph value on the retention of ionic compounds was investigated for understanding the chromatographic behaviors. the results demonstrated that click te-gsh column could provide both hydrophilic and cation-exchange interaction. taking advantage of the good hydrophilicity and inherent cation-exchange characteristics of click te-gsh material, the resolution of neutral fructosan with high degree of polymerization (dp), basic chitooligosaccharides and strongly acidic carrageenan oligosaccharides was successfully realized in hydrophilic interaction chromatography (hilic), hydrophilic interaction/cation-exchange mixed-mode chromatography (hilic/cex), cation-exchange chromatography (cex) and electrostatic repulsion/hydrophilic interaction chromatography (erlic). on the other hand, the separation of standard peptides varying in hydrophobicity/hydrophilicity and charge was achieved in both cex and hilic/cex mode with high efficiency and distinct selectivity. to further demonstrate the versatility and applicability of click te-gsh stationary phase, the separation of a human serum albumin (hsa) tryptic digest was performed in hilic/cex mode. peptides were adequately resolved and up to 86 hsa peptides were identified with sequence coverage of 85%. the results indicated the good potential of click te-gsh material in glycomics and proteomics. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | interaction liquid-chromatography ; electrospray mass-spectrometry ; polar compounds ; reversed-phase ; click chemistry ; drug discovery ; separation ; peptides ; strategy ; histones |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325841300009 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137685]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing 100864, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Aijin,Li, Xiuling,Dong, Xuefang,et al. Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography[J]. journal of chromatography a,2013,1314:63-69. |
| APA | Shen, Aijin,Li, Xiuling,Dong, Xuefang,Wei, Jie,Guo, Zhimou,&Liang, Xinmiao.(2013).Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography.journal of chromatography a,1314,63-69. |
| MLA | Shen, Aijin,et al."Glutathione-based zwitterionic stationary phase for hydrophilic interaction/cation-exchange mixed-mode chromatography".journal of chromatography a 1314(2013):63-69. |
入库方式： OAI收割
来源：大连化学物理研究所
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