Oxygen diffusivity in alginate/chitosan microcapsules
文献类型：期刊论文
| 作者 | Zhao, Wei1,2; Zhang, Ying1; Liu, Yang1; Tan, Mingqian1; Yu, Weiting1; Xie, Hongguo1; Ma, Ying1,2; Sun, Guangwei1; Lv, Guojun1; Zhao, Shan1 |
| 刊名 | journal of chemical technology and biotechnology
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 88期号:3页码:449-455 |
| 关键词 | dissolved oxygen alginate chitosan microcapsules effective diffusion coefficient cell culture |
| 英文摘要 | background: oxygen diffusion properties affect the proliferation and metabolism of cells cultured in microcapsules with a polyelectrolyte complex membrane. the effective diffusion coefficient (de) of oxygen in alginate/chitosan (ac) microcapsules under different preparation conditions was calculated, and a mathematic model was developed to investigate the effect of oxygen diffusion on cell loading in the microcapsules. results: oxygen de in ac microcapsules was independent of alginate solution concentration, intrinsic viscosity of alginate and different polyelectrolyte complex membranes. de decreased from 2.1 +/- 0.3 x 109 to 0.17 +/- 0.01 x 109 m2 s1 as microcapsule diameter decreased from 1800 to 45 0 mu m. microcapsule density was increased from 1.013 +/- 0.000 to 1.034 +/- 0.003 g ml1 as diameter decreased from 1775 to 430 mu m. the mathematic model results showed that critical cho cell loadings were 1.8 x 108 or 1.1 x 108 cells ml1 in microcapsules with 450 or 1800 mu m diameter, respectively. conclusions: no significant difference was found of oxygen de between calcium alginate beads and ac microcapsules. the decrease of de with diameter was attributed to the increasing density and compact degree on the surface. the model results indicated that risk on necrosis rose with the increasing diameter. microcapsules with smaller diameters may have more advantages on cell culture. (c) 2012 society of chemical industry |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; chemistry, multidisciplinary ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; chemistry ; engineering |
| 关键词[WOS] | immobilized cell systems ; alginate gel beads ; mass-transfer ; bioartificial pancreas ; permeability ; chitosan ; membrane ; encapsulation ; stability ; growth |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315123400016 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137698]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Wei,Zhang, Ying,Liu, Yang,et al. Oxygen diffusivity in alginate/chitosan microcapsules[J]. journal of chemical technology and biotechnology,2013,88(3):449-455. |
| APA | Zhao, Wei.,Zhang, Ying.,Liu, Yang.,Tan, Mingqian.,Yu, Weiting.,...&Ma, Xiaojun.(2013).Oxygen diffusivity in alginate/chitosan microcapsules.journal of chemical technology and biotechnology,88(3),449-455. |
| MLA | Zhao, Wei,et al."Oxygen diffusivity in alginate/chitosan microcapsules".journal of chemical technology and biotechnology 88.3(2013):449-455. |
入库方式： OAI收割
来源：大连化学物理研究所
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