Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426
文献类型：期刊论文
| 作者 | Zhao, Pengchao1,2; Quan, Chunshan3; Jin, Liming3; Wang, Lina1,2; Guo, Xinjuan1,2; Fan, Shengdi3 |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 35期号:12页码:2155-2163 |
| 关键词 | Bacillomycin Bacillus amyloliquefaciens Bioinformatics analysis Fengycin Functional domains Iturin Non-ribosomal peptide synthetases Sequence analysis |
| 英文摘要 | lipopeptides secreted by bacteria attract interest because of their uses in biomedicine, biotechnology and food technology; however, harnessing their megasynthases (non-ribosomal peptide synthetases, nrpss) has met with some difficulties in heterologous expression and crystallization. here, we used similarity and phylogenetic analysis of nrps sequences, including the fengycin and iturin family synthetases from bacillus spp., and have developed a novel approach for delineating the length and boundaries of nrps domains from bacillus amyloliquefaciens strain q-426. the sequences were further characterized (including specific residues and conserved motifs) that gave insight into the basis of the substrate specificity. data from the prediction of the nrps domains, obtained by the self-optimized prediction method with alignment program, showed they are all structurally unstable, making it difficult to determine their crystal structures. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | thioesterase domain ; adenylation domains ; structural basis ; gramicidin-s ; surfactin ; evolution ; recognition ; cyclization ; daptomycin ; prediction |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326631100025 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137708]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Nationalities Univ, Dept Life Sci, Dalian 116600, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Pengchao,Quan, Chunshan,Jin, Liming,et al. Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426[J]. biotechnology letters,2013,35(12):2155-2163. |
| APA | Zhao, Pengchao,Quan, Chunshan,Jin, Liming,Wang, Lina,Guo, Xinjuan,&Fan, Shengdi.(2013).Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426.biotechnology letters,35(12),2155-2163. |
| MLA | Zhao, Pengchao,et al."Sequence characterization and computational analysis of the non-ribosomal peptide synthetases controlling biosynthesis of lipopeptides, fengycins and bacillomycin D, from Bacillus amyloliquefaciens Q-426".biotechnology letters 35.12(2013):2155-2163. |
入库方式： OAI收割
来源：大连化学物理研究所
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