Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel
文献类型：期刊论文
| 作者 | Yu, Xiaoming1,2; Zhao, Peng2; Zhang, Lihua2; Zhang, Yukui2 |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 27期号:11页码:1574-1579 |
| 关键词 | target proteins screening polyacrylamide cryogel monolith phage-displayed cDNA libary biopanning |
| 英文摘要 | monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage-displayed human liver cdna library. the macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an spe cartridge, phage clones that displayed homo sapiens methyl cpg binding protein 2 (mecp2) were selectively enriched. the interaction between doxorubicin and mecp2 displayed phages was further validated by studying the retention of doxorubicin on mecp2 phage-coupled cryogel. these results demonstrate that drug-coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage-displayed library. copyright (c) 2013 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | dna methyltransferases ; affinity ligands ; binding ; protein ; identification ; purification ; adsorbents ; chromatography ; exchange ; capture |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328324100028 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137722]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Xiaoming,Zhao, Peng,Zhang, Lihua,et al. Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel[J]. biomedical chromatography,2013,27(11):1574-1579. |
| APA | Yu, Xiaoming,Zhao, Peng,Zhang, Lihua,&Zhang, Yukui.(2013).Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel.biomedical chromatography,27(11),1574-1579. |
| MLA | Yu, Xiaoming,et al."Screening of phage-displayed human liver cDNA library against doxorubicin with drug-immobilized monolithic polyacrylamide cryogel".biomedical chromatography 27.11(2013):1574-1579. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。