Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry
文献类型：期刊论文
| 作者 | Chen, Shili1; Hoene, Miriam2,3; Li, Jia1; Li, Yanjie1; Zhao, Xinjie1; Haering, Hans-Ulrich3,4; Schleicher, Erwin D.2,3; Weigert, Cora2,3; Xu, Guowang1; Lehmann, Rainer2,3 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-07-12 |
| 卷号 | 1298页码:9-16 |
| 关键词 | Liquid chromatography/mass spectrometry Metabolic profiling Sample pretreatment Methyl tert-butyl ether (MTBE) Biopsy Metabolomics |
| 英文摘要 | a common challenge for scientists working with animal tissue or human biopsy samples is the limitation of material and consequently, the difficulty to perform comprehensive metabolic profiling within one experiment. here, we present a novel approach to simultaneously perform targeted and non-targeted metabolomics as well as lipidomics from one small piece of liver or muscle tissue by ultra-high performance liquid chromatography/mass spectrometry (uhplc/ms) following a methyl tert-butyl ether (mtbe)-based extraction. equal relative amounts of the resulting polar and non-polar fractions were pooled, evaporated and reconstituted in the appropriate solvent for uhplc/ms analysis. this mix was comparable or superior in yield and reproducibility to a standard 80% methanol extraction for the profiling of polar and lipophilic metabolites (free carnitine, acylcarnitines and ffa). the mix was also suitable for non-targeted metabolomics, an easy measure to increase the metabolite coverage by 30% relative to using the polar fraction alone. lipidomics was performed from an aliquot of the non-polar fraction. this novel strategy could successfully be applied to one mouse soleus muscle with a dry weight of merely 2.5 mg. by enabling a simultaneous profiling of lipids and metabolites with mixed polarity while saving material for molecular, biochemical or histological analyses, our approach may open up new perspectives toward a comprehensive investigation of small, valuable tissue samples. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | fatty-acid-composition ; mass-spectrometry ; muscle ; resistance ; disease ; plasma ; brain ; liver ; serum ; model |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321174700002 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137725]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Hosp Tuebingen, Dept Internal Med 4, Div Clin Chem & Pathobiochem, Tubingen, Germany 3.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Paul Langerhans Inst Tuebingen, Tubingen, Germany 4.Univ Hosp Tuebingen, Dept Internal Med 4, Div Endocrinol Diabetol Vasc Med Nephrol & Clin C, Tubingen, Germany |
| 推荐引用方式 GB/T 7714 | Chen, Shili,Hoene, Miriam,Li, Jia,et al. Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry[J]. journal of chromatography a,2013,1298:9-16. |
| APA | Chen, Shili.,Hoene, Miriam.,Li, Jia.,Li, Yanjie.,Zhao, Xinjie.,...&Lehmann, Rainer.(2013).Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry.journal of chromatography a,1298,9-16. |
| MLA | Chen, Shili,et al."Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry".journal of chromatography a 1298(2013):9-16. |
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来源：大连化学物理研究所
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