Quantification of endogenous brassinosteroids in plant by on-line two-dimensional microscale solid phase extraction-on column derivatization coupled with high performance liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Wu, Qian1,2; Wu, Dapeng1; Shen, Zheng1; Duan, Chunfeng1; Guan, Yafeng1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-07-05 |
| 卷号 | 1297页码:56-63 |
| 关键词 | On-line two-dimensional microscale solid phase extraction (2D mu SPE) On-column derivatization (OCD) Endogenous brassinosteroids (BRs) Tomato leaf sample |
| 英文摘要 | an on-line two-dimensional microscale solid phase extraction (2d mu spe)-on column derivatization (ocd)-high performance liquid chromatography coupled with tandem mass spectrometry (hplc-ms/ms) method was developed for quantification of brassinosteroids (brs) in plant tissues. five brs with widest distribution in plant species and high bioactivity (24-epibrassinolide,24-epicastasterone, 6-deoxo-24-epicastasterone, teasterone and typhastero) were selected as target analytes. 2d mu spe column packed sequentially with phenyl boronic acid silica sorbent (the first dimension) and c18 silica sorbent (the second dimension) was used to selectively extract and enrich brs by 110-146 times. ocd was carried out on the second dimension of 2d mu spe column with m-aminophenylboronic acid (m-apba) as a derivatization reagent, enhancing the sensitivity of ms/ms to brs by 13-8437 times. it was also found that pre-trap of derivatization reagent on the c18 section of 2d mu spe column could increase reaction efficiency by 3-10 times. the whole process time of the on-line system was less than 30 min. the detection limits of the method for five brs were between 1.4 and 6.6 pg with rsds less than 10%. endogeneous brs in tomato leaves were analyzed by using this method. owing to the high selectivity of this on-line 2d mu spe system, brs in plant extracts could be quantified using matrix-free standard calibration method with relative recoveries in the range of 80-124%. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | gas-chromatography ; acid |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320821900007 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137747]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Qian,Wu, Dapeng,Shen, Zheng,et al. Quantification of endogenous brassinosteroids in plant by on-line two-dimensional microscale solid phase extraction-on column derivatization coupled with high performance liquid chromatography-tandem mass spectrometry[J]. journal of chromatography a,2013,1297:56-63. |
| APA | Wu, Qian,Wu, Dapeng,Shen, Zheng,Duan, Chunfeng,&Guan, Yafeng.(2013).Quantification of endogenous brassinosteroids in plant by on-line two-dimensional microscale solid phase extraction-on column derivatization coupled with high performance liquid chromatography-tandem mass spectrometry.journal of chromatography a,1297,56-63. |
| MLA | Wu, Qian,et al."Quantification of endogenous brassinosteroids in plant by on-line two-dimensional microscale solid phase extraction-on column derivatization coupled with high performance liquid chromatography-tandem mass spectrometry".journal of chromatography a 1297(2013):56-63. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。