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Chinese Academy of Sciences Institutional Repositories Grid
The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells

文献类型:期刊论文

作者Zhang, Qiaoyin1; Huang, Nianyu1; Wang, Junzhi1,2; Luo, Huajun1; He, Haibo1; Ding, Mingruo1; Deng, Wei-Qiao3; Zou, Kun1
刊名fitoterapia
出版日期2013-09-01
卷号89页码:210-217
关键词Trametenolic acid B H+/K+-ATPase inhibitory activity Docking simulation Anti-gastric cancer
英文摘要trametenolic acid b (tab), the bioactive component in the trametes lactinea (berk.) pat, was reported to possess cytotoxic activities and thrombin inhibiting effects. this study was performed to investigate the effects of tab on h+/k+-atpase and gastric cancer. the h+/k+-atpase inhibitory activity was determined by gastric parietal cells. compared to the normal control group, tab (10, 20, 40 and 80 ug/ml) inhibited the h+/k+-atpase activity by 15.97, 16.96, 24.86 and 16.25%, respectively. in the study, 36 kunming mice were randomly divided into six groups: control, model, tab-l (tab, 5 mg/kg/day, i.g.), tab-m (tab, 20 mg/kg/day, i.g.), tab-h (tab, 40 mg/kg/day, i.g.) and omeprazole (ol, 10 mg/kg/day, i.g.). all mice except the control group were administrated with anhydrous alcohol (5.0 ml/kg, i.g.) for induced gastric-ulcer 1 h after the 5th day. at the same time, the control mice were given the same volume of physiological saline. after 4 h, tab was evaluated for h+/k+-atpase inhibitory activities of ulcerative gaster, gastric ulcer index and ulcer inhibition. in vitro, the anti-proliferation effect of tab to gastric cancer cell (hgc-27) in acid environment was detected by mtt, and the apoptosis morphological changes were also observed by hoechst 33258 dye assay. the results indicated that tab inhibited moderately h+/k+-atpase activity in vitro. compared to the model group, tab showed anti-ulcer effects in gastric tissue with the dosages of 20 and 5 mg/kg in vivo. apart from that, tab could selectively inhibit gastric cancer cell viability and reduce cell apoptosis against hgc-27 cells at low doses in acid environment. (c) 2013 the authors. published by elsevier b.v. all rights reserved.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]chemistry, medicinal ; pharmacology & pharmacy
研究领域[WOS]pharmacology & pharmacy
关键词[WOS]proton pump inhibitor ; peptic-ulcer disease ; helicobacter-pylori ; inonotus-obliquus ; in-vitro ; h+,k+-atpase ; triterpenes ; apoptosis ; vivo
收录类别SCI
语种英语
WOS记录号WOS:000323856500024
公开日期2015-11-10
源URL[http://159.226.238.44/handle/321008/137755]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.China Three Gorges Univ, Hubei Key Lab Nat Prod Res & Dev, Yichang 443002, Peoples R China
2.Hubei Tujia Inst Med, Yichang 443002, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Qiaoyin,Huang, Nianyu,Wang, Junzhi,et al. The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells[J]. fitoterapia,2013,89:210-217.
APA Zhang, Qiaoyin.,Huang, Nianyu.,Wang, Junzhi.,Luo, Huajun.,He, Haibo.,...&Zou, Kun.(2013).The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells.fitoterapia,89,210-217.
MLA Zhang, Qiaoyin,et al."The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells".fitoterapia 89(2013):210-217.

入库方式: OAI收割

来源:大连化学物理研究所

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