The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells
文献类型:期刊论文
作者 | Zhang, Qiaoyin1; Huang, Nianyu1; Wang, Junzhi1,2; Luo, Huajun1; He, Haibo1; Ding, Mingruo1; Deng, Wei-Qiao3; Zou, Kun1 |
刊名 | fitoterapia
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出版日期 | 2013-09-01 |
卷号 | 89页码:210-217 |
关键词 | Trametenolic acid B H+/K+-ATPase inhibitory activity Docking simulation Anti-gastric cancer |
英文摘要 | trametenolic acid b (tab), the bioactive component in the trametes lactinea (berk.) pat, was reported to possess cytotoxic activities and thrombin inhibiting effects. this study was performed to investigate the effects of tab on h+/k+-atpase and gastric cancer. the h+/k+-atpase inhibitory activity was determined by gastric parietal cells. compared to the normal control group, tab (10, 20, 40 and 80 ug/ml) inhibited the h+/k+-atpase activity by 15.97, 16.96, 24.86 and 16.25%, respectively. in the study, 36 kunming mice were randomly divided into six groups: control, model, tab-l (tab, 5 mg/kg/day, i.g.), tab-m (tab, 20 mg/kg/day, i.g.), tab-h (tab, 40 mg/kg/day, i.g.) and omeprazole (ol, 10 mg/kg/day, i.g.). all mice except the control group were administrated with anhydrous alcohol (5.0 ml/kg, i.g.) for induced gastric-ulcer 1 h after the 5th day. at the same time, the control mice were given the same volume of physiological saline. after 4 h, tab was evaluated for h+/k+-atpase inhibitory activities of ulcerative gaster, gastric ulcer index and ulcer inhibition. in vitro, the anti-proliferation effect of tab to gastric cancer cell (hgc-27) in acid environment was detected by mtt, and the apoptosis morphological changes were also observed by hoechst 33258 dye assay. the results indicated that tab inhibited moderately h+/k+-atpase activity in vitro. compared to the model group, tab showed anti-ulcer effects in gastric tissue with the dosages of 20 and 5 mg/kg in vivo. apart from that, tab could selectively inhibit gastric cancer cell viability and reduce cell apoptosis against hgc-27 cells at low doses in acid environment. (c) 2013 the authors. published by elsevier b.v. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | chemistry, medicinal ; pharmacology & pharmacy |
研究领域[WOS] | pharmacology & pharmacy |
关键词[WOS] | proton pump inhibitor ; peptic-ulcer disease ; helicobacter-pylori ; inonotus-obliquus ; in-vitro ; h+,k+-atpase ; triterpenes ; apoptosis ; vivo |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000323856500024 |
公开日期 | 2015-11-10 |
源URL | [http://159.226.238.44/handle/321008/137755] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.China Three Gorges Univ, Hubei Key Lab Nat Prod Res & Dev, Yichang 443002, Peoples R China 2.Hubei Tujia Inst Med, Yichang 443002, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Qiaoyin,Huang, Nianyu,Wang, Junzhi,et al. The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells[J]. fitoterapia,2013,89:210-217. |
APA | Zhang, Qiaoyin.,Huang, Nianyu.,Wang, Junzhi.,Luo, Huajun.,He, Haibo.,...&Zou, Kun.(2013).The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells.fitoterapia,89,210-217. |
MLA | Zhang, Qiaoyin,et al."The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells".fitoterapia 89(2013):210-217. |
入库方式: OAI收割
来源:大连化学物理研究所
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