Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Zhuo, Rongjie1; Zhang, Liangxiao1; Wang, Li1; Shan, Guoshun1,2; Yang, Qianxu1; Yuan, Xiaoyan1; Xiao, Hongbin1 |
| 刊名 | analytical methods
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:8页码:2046-2052 |
| 英文摘要 | a rapid and sensitive method for simultaneous quantitative determination of terpenoids in tripterygium wilfordii hook f (twhf) was developed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (uhplc-qtof-ms). in this method, five major terpenoids, including triptolide, triptonide, demethylzelastral, tripterine and wilforlide a, with large content range in twhf, were determined simultaneously in a uhplc run of 4.5 minutes. the intra-day and inter-day precisions of five terpenoids were less than 4.58% and 4.82%, respectively, and the mean recovery rates obtained were in the range of 97.2% to 104.5%. all calibration curves showed good linearity (r(2) > 0.996) from 20 ng ml(-1) and 20 000 ng ml(-1). the limit of detection (lod) and limit of quantitation (loq) were less than 5.7 ng ml(-1) and 19.0 ng ml(-1). compared with previous studies, this method could be employed for accurate quantification of five terpenoids in different parts and products of twhf in a very short time after simple ultrasonic extraction. the results indicated that this method would provide a rapid and sensitive way for evaluating the quality of the twhf and its products. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine ; technology |
| 类目[WOS] | chemistry, analytical ; food science & technology ; spectroscopy |
| 研究领域[WOS] | chemistry ; food science & technology ; spectroscopy |
| 关键词[WOS] | solid-phase extraction ; root extracts ; triptolide ; tripdiolide ; alkaloids ; triptonide ; tripterine ; leaves |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316960200022 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137820]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Liaoning Univ TCM, Sch Pharm, Dalian 116600, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuo, Rongjie,Zhang, Liangxiao,Wang, Li,et al. Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry[J]. analytical methods,2013,5(8):2046-2052. |
| APA | Zhuo, Rongjie.,Zhang, Liangxiao.,Wang, Li.,Shan, Guoshun.,Yang, Qianxu.,...&Xiao, Hongbin.(2013).Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.analytical methods,5(8),2046-2052. |
| MLA | Zhuo, Rongjie,et al."Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry".analytical methods 5.8(2013):2046-2052. |
入库方式： OAI收割
来源：大连化学物理研究所
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