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1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry

文献类型：期刊论文

作者Qiao XiaoQiang1,2,3; Zhou Yuan1; Hou ChunYan1; Zhang XiaoDan1; Yang KaiGuang1; Zhang LiHua1; Zhang YuKui1
刊名science china-life sciences
出版日期2013-03-01
卷号56期号:3页码:240-245
关键词1-(3-aminopropyl)-3-butylimidazolium bromide ionization capacity peptide mass spectrometry analysis derivatization
英文摘要the cationic reagent 1-(3-aminopropyl)-3-butylimidazolium bromide (bapi) was exploited for the derivatization of carboxyl groups on peptides. nearly 100% derivatization efficiency was achieved with the synthetic peptide rvyvhpi (ri-7). furthermore, the peptide derivative was stable in a 0.1% tfa/water solution or a 0.1% (v/v) tfa/acetonitrile/water solution for at least one week. the effect of bapi derivatization on the ionization of the peptide ri-7 was further investigated, and the detection sensitivity was improved > 42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), thus outperforming the commercial piperazine derivatization approach. moreover, the charge states of the peptide were largely increased via bapi derivatization by electrospray ionization (esi) ms. the results indicate the potential merits of bapi derivatization for high sensitivity peptide analysis by ms.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biology
研究领域[WOS]life sciences & biomedicine - other topics
关键词[WOS]chemical derivatization ; ms/ms analysis ; maldi-ms ; ionization ; phosphopeptides ; enhancement ; proteins ; capture ; liquid
收录类别SCI
语种英语
WOS记录号WOS:000316747000007
公开日期2015-11-10
源URL[http://159.226.238.44/handle/321008/137821]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hebei Univ, Key Lab Pharmaceut Qual Control Hebei Prov, Baoding 071002, Peoples R China
3.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China
推荐引用方式
GB/T 7714		 Qiao XiaoQiang,Zhou Yuan,Hou ChunYan,et al. 1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry[J]. science china-life sciences,2013,56(3):240-245.
APA Qiao XiaoQiang.,Zhou Yuan.,Hou ChunYan.,Zhang XiaoDan.,Yang KaiGuang.,...&Zhang YuKui.(2013).1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry.science china-life sciences,56(3),240-245.
MLA Qiao XiaoQiang,et al."1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry".science china-life sciences 56.3(2013):240-245.

入库方式： OAI收割

来源：大连化学物理研究所

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