Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke
文献类型：期刊论文
| 作者 | Liu, Hailin1,2; Sun, Shihao2; Zong, Yongli2; Li, Peng2; Xie, Jianping2 |
| 刊名 | analytical letters
 |
| 出版日期 | 2013-02-15 |
| 卷号 | 46期号:4页码:682-693 |
| 关键词 | Antioxidants Cigarette smoke Reactive oxygen species Spectrofluorometer |
| 英文摘要 | cigarette smoke can cause cellular oxidative stress that contributes to various adverse health effects associated with smoke exposure, partially due to reactive oxygen species (ros) present in cigarette smoke. reduction of abundant ros in the cigarette mainstream smoke (mss) is of importance for human health. in this work, a simple, rapid, and reliable fluorescence evaluation of scavenging efficiency of antioxidants as potential filter additives against ros in cigarette smoke is reported. this method was based on the combination of model glass reactor and a fluorescence assay of ros in cigarette smoke using dihydrorhodamine 6g (dhr-6g). the antioxidant was added into a glass reactor attached to cigarette filter, which simplified the preparation of combined filter containing additives. the ros scavenging efficiency of antioxidants was then determined using spectrofluorimetry by the change in fluorescence intensity of whole smoke-bubbled solutions before and after addition of antioxidants into the glass reactor. the proposed method was successfully applied to the determination of ros scavenging efficiency of several potential additives, such as tert-butylhydroquinone (tbhq), vitamin c, -carotene, grape seed extract, and ginkgo biloba extract. moreover, the relationship between mss ros scavenging efficiency and antioxidant activities (dpph radicals scavenging efficiency and fe2+ reducing power) of these compounds was also investigated. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | free-radicals ; tobacco-smoke ; n-nitrosamines ; beta-carotene ; in-vivo ; mainstream ; filters ; reduce ; 2,2-diphenyl-1-picrylhydrazyl ; resonance |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315156700011 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137826]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hailin,Sun, Shihao,Zong, Yongli,et al. Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke[J]. analytical letters,2013,46(4):682-693. |
| APA | Liu, Hailin,Sun, Shihao,Zong, Yongli,Li, Peng,&Xie, Jianping.(2013).Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke.analytical letters,46(4),682-693. |
| MLA | Liu, Hailin,et al."Fluorescence Evaluation of Scavenging Efficiency of Antioxidants Against Reactive Oxygen Species (ROS) in Cigarette Smoke".analytical letters 46.4(2013):682-693. |
入库方式： OAI收割
来源：大连化学物理研究所
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