Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20
文献类型：期刊论文
| 作者 | Huang, Lishuxin1,2; Zhou, Jungang3; Li, Xiao4; Peng, Qiang1,2; Lu, Hong3; Du, Yuguang1 |
| 刊名 | journal of industrial microbiology & biotechnology
 |
| 出版日期 | 2013 |
| 卷号 | 40期号:1页码:113-122 |
| 关键词 | Flavobacterium Alginate lyase Saccharification Large-scale preparation Alginate oligosaccharides |
| 英文摘要 | alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of biochemicals and renewable biofuels. this study described the isolation of a new alginate metabolizing bacterium, flavobacterium sp. s20, from sludge samples and the characterization of its alginate lyase alg2a. the alginate lyase gene, alg2a, was obtained by constructing and screening the genomic library of the strain s20 and overexpressed in escherichia coli. substrate specificity assays indicated alg2a preferred poly-alpha-l-guluronate as a substrate over poly-beta-d-mannuronate. in the saccharification process of a high content (10 %, w/v) of sodium alginate, the recombinant alginate lyase alg2a yielded 152 of mm the reducing sugars after 69 h of reaction, and the amounts of oligosaccharides with a different degree of polymerization (dp) generated by alg2a gradually accumulated without significant variation in the distribution of oligosaccharide compositions. these results indicated that alg2a possessed high enzymatic capability for saccharifying the alginate, which could be used in saccharifying the alginate biomass prior to the main fermentation process for biofuels. in addition, alg2a had a different endolytic reaction mode from both the two commercial alginate lyases and other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of alg2a. thus, alg2a could be a good tool for the large-scale preparation of alginate oligosaccharides with high dp. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | saccharophagus degradans ; pseudomonas-aeruginosa ; oligoalginate lyase ; escherichia-coli ; guluronate lyase ; purification ; acid ; oligosaccharides ; overexpression ; sugar |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313019300012 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137931]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Liaoning Prov Key Lab Carbohydrates,CAS, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China 4.NW Univ Xian, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Shan Xi Prov Key Lab Biotechnol,Life Sci Coll, Xian 710069, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Lishuxin,Zhou, Jungang,Li, Xiao,et al. Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20[J]. journal of industrial microbiology & biotechnology,2013,40(1):113-122. |
| APA | Huang, Lishuxin,Zhou, Jungang,Li, Xiao,Peng, Qiang,Lu, Hong,&Du, Yuguang.(2013).Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20.journal of industrial microbiology & biotechnology,40(1),113-122. |
| MLA | Huang, Lishuxin,et al."Characterization of a new alginate lyase from newly isolated Flavobacterium sp S20".journal of industrial microbiology & biotechnology 40.1(2013):113-122. |
入库方式： OAI收割
来源：大连化学物理研究所
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