Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides
文献类型：期刊论文
| 作者 | Cao, Liwei; Yu, Long; Guo, Zhimou; Li, Xiuling; Xue, Xinya; Liang, Xinmiao |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-07-19 |
| 卷号 | 1299页码:18-24 |
| 关键词 | SAX ERLIC Glycopeptides Enrichment Mass spectrometry |
| 英文摘要 | glycoproteins are involved in various cellular activities, including inter- and extracellular signaling. however, glycopeptide signals are significantly suppressed by coeluting non-glycosylated peptides in mass spectrometry-based analysis. for detailed elucidation of the biological functions of glycoproteins, selective enrichment of glycopeptides from non-glycosylated peptides is crucial. in the present study, a sax material, xcharge sax, was used in a column in the erlic mode with the aim of specifically enriching glycopeptides. enrichment conditions were initially optimized, and selectivity, glycosylation heterogeneity coverage and detection sensitivity of xcharge sax were subsequently assessed. in the selectivity assessment, glycopeptides were effectively isolated from a peptide mixture (human serum immunoglobulin g (igg) and human serum albumin digests) and a tryptic digest of human serum using xcharge sax. in the evaluation of glycosylation heterogeneity coverage, five glycosites and eleven glyco peptides from horseradish peroxidase were identified after enrichment with xcharge sax. in detection sensitivity assessment, glycopeptides within four orders of magnitude were identified after enrichment with xcharge sax. in addition, volatile solvents were used in the loading and eluting buffers so that desalting was not necessary for erlic fractions. our results collectively support the utility of xcharge sax as a suitable chromatographic material for global glycosylation site analysis. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | n-linked glycoproteins ; tandem mass-spectrometry ; solid-phase extraction ; glycosylation heterogeneity ; biomarker discovery ; affinity capture ; facile synthesis ; identification ; separation ; database |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321424000003 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/137932]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Liwei,Yu, Long,Guo, Zhimou,et al. Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides[J]. journal of chromatography a,2013,1299:18-24. |
| APA | Cao, Liwei,Yu, Long,Guo, Zhimou,Li, Xiuling,Xue, Xinya,&Liang, Xinmiao.(2013).Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides.journal of chromatography a,1299,18-24. |
| MLA | Cao, Liwei,et al."Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides".journal of chromatography a 1299(2013):18-24. |
入库方式： OAI收割
来源：大连化学物理研究所
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