Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.
文献类型：期刊论文
| 作者 | Jiang, SM ; Sun, W ; Chen, MJ ; Dai, SK ; Zhang, L ; Liu, YH ; Lee, KJ ; Li, X |
| 刊名 | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 92期号:4页码:405 |
| 关键词 | marine sponge actinobacteria 16S rRNA RFLP PKS NRPS |
| ISSN号 | 0003-6072 |
| 通讯作者 | lixiang@scsio.ac.cn |
| 合作状况 | This study describes actinobacteria isolated from the marine sponge Haliclona sp. collected in shallow water of the South China Sea. A total of 54 actinobacteria were isolated using media selective for actinobacteria. Species diversity and natural product diversity of isolates from marine sponge Haliclona sp. were analysed. Twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum Actinobacteria by a combination of 16S rRNA gene based restriction enzymes digestion and 16S rRNA gene sequence analysis. The 16S rRNA genes of 24 isolates were digested by restriction enzymes TaqI and MspI and assigned to different groups according to their restriction enzyme pattern. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Streptomyces, Nocardiopsis, Micromonospora and Verrucosispora; one other isolate was recovered that does not belong to known genera based on its unique 16S rRNA gene sequence. To our knowledge, this is the first report of a bacterium classified as Verrucosispora sp. that has been isolated from a marine sponge. The majority of the strains tested belong to the genus Streptomyces and three isolates may be new species. All of the 24 isolates were screened for genes encoding polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). PKS and NRPS sequences were detected in more than half of the isolates and the different "PKS-I-PKS-II-NRPS" combinations in different isolates belonging to the same species are indicators of their potential natural product diversity and divergent genetic evolution. |
| 收录类别 | 416 |
| 原文出处 | - |
| 公开日期 | 2010-11-04 ; 2011-04-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/1079]  |
| 专题 | 南海海洋研究所_广东省应用海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Jiang, SM,Sun, W,Chen, MJ,et al. Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2007,92(4):405. |
| APA | Jiang, SM.,Sun, W.,Chen, MJ.,Dai, SK.,Zhang, L.,...&Li, X.(2007).Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp..ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,92(4),405. |
| MLA | Jiang, SM,et al."Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 92.4(2007):405. |
入库方式： OAI收割
来源：南海海洋研究所
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